TY - GEN AB - There is a surprisingly large gap between the urgency of understanding the basic biology of pathogenic viruses and the tools and strategies that have been brought to bear. Unraveling the host dependency factors that support viral pathogenesis, and identifying viral factors that interact with the host to facilitate their life cycles, is the basis of our ability to develop antiviral strategies. The fundamental hypothesis guiding my dissertation work has been that (+)-sense viruses of the flavivirus and coronavirus genre manipulate host lipid metabolism, and further, that this lipid remodeling is a critical part of the viral life cycle. By necessity, this has been an extremely interdisciplinary project, ranging from chemical synthesis of relevant lipid probes, through the analytic chemistry techniques of lipidomics and proteomics, ultimately applied to and in conversation with virology. AU - Farley, Scotland E. DA - 2023 DO - 10.6083/3484zh75r DO - DOI ID - 10138 KW - Biology KW - Host-Pathogen Interactions KW - Lipidomics KW - Lipids KW - Biochemistry KW - Virology L1 - https://digitalcollections.ohsu.edu/record/10138/files/Farley.Scotland.2023.pdf L2 - https://digitalcollections.ohsu.edu/record/10138/files/Farley.Scotland.2023.pdf L4 - https://digitalcollections.ohsu.edu/record/10138/files/Farley.Scotland.2023.pdf LK - https://digitalcollections.ohsu.edu/record/10138/files/Farley.Scotland.2023.pdf N2 - There is a surprisingly large gap between the urgency of understanding the basic biology of pathogenic viruses and the tools and strategies that have been brought to bear. Unraveling the host dependency factors that support viral pathogenesis, and identifying viral factors that interact with the host to facilitate their life cycles, is the basis of our ability to develop antiviral strategies. The fundamental hypothesis guiding my dissertation work has been that (+)-sense viruses of the flavivirus and coronavirus genre manipulate host lipid metabolism, and further, that this lipid remodeling is a critical part of the viral life cycle. By necessity, this has been an extremely interdisciplinary project, ranging from chemical synthesis of relevant lipid probes, through the analytic chemistry techniques of lipidomics and proteomics, ultimately applied to and in conversation with virology. PB - Oregon Health and Science University PY - 2023 T1 - Disentangling the web: the synthesis of functional lipid probes to study metabolic rewiring in RNA virus infection TI - Disentangling the web: the synthesis of functional lipid probes to study metabolic rewiring in RNA virus infection UR - https://digitalcollections.ohsu.edu/record/10138/files/Farley.Scotland.2023.pdf Y1 - 2023 ER -