000000146 001__ 146
000000146 005__ 20260423083220.0
000000146 0247_ $$2DOI$$a10.6083/M4445JDB
000000146 037__ $$aETD
000000146 245__ $$aThe electronic structure of the Tyr-Cys free radical in galactose oxidase determined by EPR spectroscopy
000000146 260__ $$bOregon Health and Science University
000000146 269__ $$a2007
000000146 336__ $$aThesis
000000146 502__ $$bM.S.
000000146 520__ $$aThe EPR spectrum of the Tyr–Cys· free radical in oxidized apoGAOX was investigated using power saturation analysis and isotopic labeling. Global fitting with evolving factor analysis revealed two distinct radical species, with the dominant low-power component assigned to the Tyr–Cys· side chain. EPR spectra of multiple isotopically labeled variants enabled determination of g‑values, hyperfine coupling constants, and linewidths through spectral simulation. The results indicate significant spin density on methylene protons, aromatic carbons, and sulfur and oxygen atoms, providing detailed structural insight into the Tyr–Cys· radical.
000000146 540__ $$fCC BY
000000146 542__ $$fIn copyright - single owner
000000146 650__ $$aGalactose Oxidase$$019347
000000146 650__ $$aFree Radicals$$019268
000000146 650__ $$aElectron Spin Resonance Spectroscopy$$018295
000000146 6531_ $$atyr-cys free radical
000000146 691__ $$aOGI School of Science and Engineering$$041365
000000146 692__ $$aDepartment of Environmental and Biomolecular Systems$$041414
000000146 7001_ $$aLee, Yuk$$uOregon Health and Science University$$041354
000000146 7201_ $$aWhittaker, James$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000146 8564_ $$9decd3266-1459-4d88-ab9b-6f4e28a91599$$s1790908$$uhttps://digitalcollections.ohsu.edu/record/146/files/146_etd.pdf$$ePublic$$227cbab9a5750c672dae1479a4d5a19c3$$31
000000146 901__ $$a<p>These documents are archival records. They are retained for historical reference only. </p><p><b>Need an accessible version? Use the ‘Get Accessible Copy’ link above.</b></p>
000000146 905__ $$a/rest/prod/b8/51/5n/36/b8515n36q
000000146 909CO $$ooai:digitalcollections.ohsu.edu:146$$pstudent-work
000000146 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000146 980__ $$aTheses and Dissertations