000000200 001__ 200
000000200 005__ 20260304135025.0
000000200 0247_ $$2DOI$$a10.6083/M44T6G9F
000000200 037__ $$aETD
000000200 245__ $$aUsing optical methods to monitor and administer photodynamic therapy to oral bacteria
000000200 260__ $$bOregon Health and Science University
000000200 269__ $$a2008
000000200 336__ $$aDissertation
000000200 502__ $$bPh.D.
000000200 520__ $$aOral diseases such as periodontal disease and dental caries affect more than 75% of U.S. adults over age 35, and treatment is increasingly challenged by antibiotic‑resistant bacteria in biofilms. Photodynamic therapy (PDT), which uses light‑activated photosensitizers (PS) to kill bacteria, offers a promising alternative because it targets infected sites precisely and is unlikely to induce resistance. This study used optical methods, including reflectance spectroscopy and confocal microscopy, to characterize PS uptake and PDT dosimetry in planktonic and biofilm bacteria. The lethal PDT dose was similar in both states, but biofilms required ten‑fold higher light exposure due to limited PS penetration. A Monte Carlo tooth model was used to evaluate light delivery throughout dental structures. These findings provide essential tools and dose parameters for designing effective PDT protocols for oral biofilm infections.
000000200 540__ $$fCC BY
000000200 542__ $$fIn copyright - single owner
000000200 650__ $$aPhotochemotherapy$$024078
000000200 650__ $$aDental Caries$$017484
000000200 650__ $$aBiofilms$$030801
000000200 650__ $$aPhotosensitizing Agents$$029873
000000200 650__ $$aAnti-Bacterial Agents$$014846
000000200 650__ $$aPeriodontal Diseases$$023822
000000200 650__ $$aMonte Carlo Method$$022421
000000200 650__ $$aMicroscopy, Confocal$$030950
000000200 650__ $$aBiofilms$$030801
000000200 6531_ $$aphotodynamic therapy
000000200 6531_ $$alight propagation
000000200 691__ $$aOGI School of Science and Engineering$$041365
000000200 692__ $$aDepartment of Biomedical Engineering$$041397
000000200 7001_ $$aFu, Yongji$$uOregon Health and Science University$$041354
000000200 7201_ $$aJacques, Steven$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000200 8564_ $$907bc434d-542f-49d5-95cd-e6d63d870b2d$$s2577440$$uhttps://digitalcollections.ohsu.edu/record/200/files/200_etd.pdf$$ePublic$$22f92e539b7c6b295bca2df784889e3fb$$31
000000200 905__ $$a/rest/prod/7w/62/f8/20/7w62f8209
000000200 909CO $$ooai:digitalcollections.ohsu.edu:200$$pstudent-work
000000200 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000200 980__ $$aTheses and Dissertations