000002154 001__ 2154
000002154 005__ 20260429175304.0
000002154 0247_ $$2DOI$$a10.6083/M4H993F2
000002154 037__ $$aETD
000002154 245__ $$aMutagenic consequences of replication bypass of N3 uracil and N[superscript 2] guanine DNA adducts
000002154 260__ $$bOregon Health and Science University
000002154 269__ $$a2006
000002154 336__ $$aDissertation
000002154 502__ $$bPh.D.
000002154 502__ $$gMolecular Microbiology &amp; Immunology (sunsetting)
000002154 520__ $$aDNA is continuously exposed to endogenous and exogenous agents that form DNA adducts, which can promote mutagenesis and carcinogenesis. This dissertation examined the mutagenicity and replication bypass of site‑specifically introduced DNA adducts derived from butadiene, vinyl chloride, 4‑hydroxynonenal, and crotonaldehyde. Using mammalian single‑stranded shuttle vectors, these lesions were shown to induce error‑prone replication with lesion‑specific mutation frequencies. Butadiene‑derived N3‑deoxyuridine adducts were the most mutagenic and required translesional synthesis by polymerases η and ζ for efficient bypass. Collectively, these findings elucidate mechanisms by which structurally distinct DNA adducts contribute to mutagenesis in mammalian cells.
000002154 540__ $$fCC BY
000002154 542__ $$fIn copyright - single owner
000002154 650__ $$aButadienes$$015921
000002154 650__ $$aAldehydes$$014422
000002154 650__ $$aMutagenesis$$029042
000002154 650__ $$aVinyl Chloride$$027824
000002154 650__ $$aDNA Replication$$017991
000002154 650__ $$aDNA Adducts$$031053
000002154 650__ $$aDNA-Directed DNA Polymerase$$017989
000002154 691__ $$aSchool of Medicine$$041369
000002154 692__ $$aDepartment of Molecular Microbiology and Immunology$$041429
000002154 7001_ $$aFernandes, Priscilla$$uOregon Health and Science University$$041354
000002154 7201_ $$aLloyd, Stephen$$uOregon Health and Science University$$041354$$7Personal$$eMentor
000002154 8564_ $$97dd23df1-757c-42ab-9d49-86b47a81c690$$s5136096$$uhttps://digitalcollections.ohsu.edu/record/2154/files/2883_etd.pdf$$ePublic$$2e7ff74de84b2d2b8feed25c13c65452a$$31
000002154 901__ $$a&lt;p&gt;These documents are archival records. They are retained for historical reference only. &lt;/p&gt;&lt;p&gt;&lt;b&gt;Need an accessible version? Use the ‘Get Accessible Copy’ link above.&lt;/b&gt;&lt;/p&gt;
000002154 905__ $$a/rest/prod/hh/63/sw/10/hh63sw105
000002154 909CO $$ooai:digitalcollections.ohsu.edu:2154$$pstudent-work
000002154 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000002154 980__ $$aTheses and Dissertations