000002200 001__ 2200
000002200 005__ 20260513082427.0
000002200 0247_ $$2DOI$$a10.6083/M4K072JR
000002200 037__ $$aETD
000002200 245__ $$aFitA and FitB, a Neisseria gonorrhoeae protein complex involved in the regulation of transcellular migration
000002200 260__ $$bOregon Health and Science University
000002200 269__ $$a2006
000002200 336__ $$aDissertation
000002200 502__ $$bPh.D.
000002200 502__ $$gMolecular Microbiology & Immunology (sunsetting)
000002200 520__ $$aNeisseria gonorrhoeae is the causative agent of gonorrhea, a common sexually transmitted infection. In this study, I characterize the fit locus and its role in host–pathogen interactions. Mutations in the fit locus increased the rate of bacterial transcytosis across polarized human cell monolayers and impaired pilE intragenic recombination during infection. The fit locus encodes two proteins, FitA and FitB. FitA is a ribbon–helix–helix DNA-binding protein that forms a heterooctomeric complex with FitB and binds a specific promoter sequence with markedly increased affinity compared to FitA alone. Structural analysis confirmed the DNA-binding mechanism and revealed a putative PIN domain in FitB. Together, these findings suggest that the fit locus promotes pilE recombination during transcytosis, contributing to immune evasion and influencing bacterial trafficking and replication.
000002200 540__ $$fCC BY
000002200 542__ $$fIn copyright - single owner
000002200 650__ $$aChemistry$$016438
000002200 650__ $$aDNA-Binding Proteins$$017998
000002200 650__ $$aGenetics$$019472
000002200 650__ $$aVesicular Transport Proteins$$034519
000002200 650__ $$aNeisseria gonorrhoeae$$022735
000002200 650__ $$aEndocytosis$$018417
000002200 650__ $$aRecombination, Genetic$$025229
000002200 650__ $$aImmune Evasion$$038852
000002200 650__ $$aGonorrhea$$019702
000002200 691__ $$aSchool of Medicine$$041369
000002200 692__ $$aDepartment of Molecular Microbiology and Immunology$$041429
000002200 7001_ $$aWilbur, John$$uOregon Health and Science University$$041354
000002200 7201_ $$aSo, Magdalene$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000002200 8564_ $$90c216d8b-1e57-468d-a094-4eac84ead33e$$s2214234$$uhttps://digitalcollections.ohsu.edu/record/2200/files/2929_etd.pdf$$ePublic$$20d64edd044f1cb8c19a627cc601e871a$$31
000002200 901__ $$a<p>These documents are archival records. They are retained for historical reference only. </p><p><b>Need an accessible version? Use the ‘Get Accessible Copy’ link above.</b></p>
000002200 905__ $$a/rest/prod/9k/41/zd/64/9k41zd64w
000002200 909CO $$ooai:digitalcollections.ohsu.edu:2200$$pstudent-work
000002200 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000002200 980__ $$aTheses and Dissertations