000002413 001__ 2413 000002413 005__ 20250424232731.0 000002413 0247_ $$2DOI$$a10.6083/M40Z71K5 000002413 037__ $$aETD 000002413 245__ $$aStructure/function studies of manganese peroxidase 000002413 260__ $$bOregon Health and Sciences University 000002413 269__ $$a2003-05-01 000002413 336__ $$aDissertation 000002413 502__ $$bPh.D. 000002413 502__ $$gBiochemistry & Molecular Biology (sunsetting) 000002413 520__ $$aLignin is a cell wall polymer that provides rigidity, cell adhesion, andmicrobial resistance to vascular plants. A sort of natural plastic, lignin contains more than 13 different randomly arranged chemical linkages and is responsible for sequestering roughly 25 % of the world's terrestrial biomass. The resulting heterogeneous three-dimensional matrix is resistant to enzymatic hydrolysis and is chemically stable under most environmental conditions. White-rot fungi are the only organisms capable of completely degrading the lignin polymer, and manganese peroxidase is a key enzyme in this process. It is the only enzyme known that selectively binds Mn2+, oxidizes it and releases Mn3+. In this work, the unique Mn- binding site of the enzyme was probed by site-directed mutagenesis. 000002413 542__ $$fIn copyright - single owner 000002413 650__ $$aMutagenesis, Site-Directed$$029043 000002413 650__ $$aPolymers$$024391 000002413 650__ $$aLignin$$021515 000002413 691__ $$aOGI School of Science and Engineering$$041365 000002413 692__ $$aDepartment of Biochemistry and Molecular Biology$$041396 000002413 7001_ $$aYoungs, Heather$$uOregon Health and Science University$$041354 000002413 7201_ $$aGold, Michael$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor 000002413 7201_ $$aLoehr, Thomas$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor 000002413 8564_ $$94d917374-ee90-4ac7-a8d1-38fbd27d052b$$s10781510$$uhttps://digitalcollections.ohsu.edu/record/2413/files/3150_etd.pdf$$ePublic$$2e3abf45c5acdc7b9319d359a5506251c$$31 000002413 905__ $$a/rest/prod/jd/47/2w/61/jd472w616 000002413 909CO $$ooai:digitalcollections.ohsu.edu:2413$$pstudent-work 000002413 980__ $$aTheses and Dissertations