TY  - GEN
AB  - This dissertation describes the development and application of a combined fluorescence and reflectance confocal microscope for in vivo cancer detection in mice through quantitative skin reflectance imaging. A novel pinhole/ring detection scheme was introduced to suppress diffuse light when imaging deep within scattering tissue, improving axial resolution by 31% compared with conventional pinhole detection. A calibration method was developed to quantify tissue reflectance relative to a mirror reference, enabling comparison across tissue types and depth. Subsurface reflectance signals exhibited exponential depth dependence, allowing derivation of optical scattering properties, including scattering coefficient (μs) and anisotropy (g), which effectively differentiated most tissue types. Using these techniques, reflectance confocal microscopy successfully imaged the initiation and progression of malignant melanoma in vivo, revealing characteristic features such as pagetoid melanocytes, tumor nests, and disruption of the dermal–epidermal junction.
AD  - Oregon Health and Science University
AU  - Gareau, Daniel
DA  - 2006
DO  - 10.6083/M4NG4NJ3
DO  - DOI
ED  - Jacques, Steven
ED  - Advisor
ID  - 258
KW  - Melanoma
KW  - Intravital Microscopy
KW  - Mice
KW  - Microscopy, Confocal
KW  - florescence microscopy
L1  - https://digitalcollections.ohsu.edu/record/258/files/258_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/258/files/258_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/258/files/258_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/258/files/258_etd.pdf
N2  - This dissertation describes the development and application of a combined fluorescence and reflectance confocal microscope for in vivo cancer detection in mice through quantitative skin reflectance imaging. A novel pinhole/ring detection scheme was introduced to suppress diffuse light when imaging deep within scattering tissue, improving axial resolution by 31% compared with conventional pinhole detection. A calibration method was developed to quantify tissue reflectance relative to a mirror reference, enabling comparison across tissue types and depth. Subsurface reflectance signals exhibited exponential depth dependence, allowing derivation of optical scattering properties, including scattering coefficient (μs) and anisotropy (g), which effectively differentiated most tissue types. Using these techniques, reflectance confocal microscopy successfully imaged the initiation and progression of malignant melanoma in vivo, revealing characteristic features such as pagetoid melanocytes, tumor nests, and disruption of the dermal–epidermal junction.
PB  - Oregon Health and Science University
PY  - 2006
T1  - In vivo confocal microscopy in turbid media
TI  - In vivo confocal microscopy in turbid media
UR  - https://digitalcollections.ohsu.edu/record/258/files/258_etd.pdf
Y1  - 2006
ER  -