000003012 001__ 3012 000003012 005__ 20250424232810.0 000003012 0247_ $$2DOI$$a10.6083/M42B8X4W 000003012 037__ $$aETD 000003012 245__ $$aThe development and use of chemical tools to identify active mycobacterial esterases 000003012 269__ $$a2016-07-01 000003012 336__ $$aDissertation 000003012 502__ $$bPh.D. 000003012 520__ $$aTuberculosis (TB) is a pulmonary disease caused by Mycobacterium tuberculosis (Mtb). Approximately one-third of the global population is infected with latent TB, an asymptomatic form of the disease that is phenotypically drug-tolerant. Dormant Mtb reactivates when the immune system weakens, contributing to the nearly 10 million new cases of active TB that occur each year. To better diagnose, treat, and ultimately eradicate TB, there is an urgent need to understand the enzymes associated with dormancy and reactivation. In my dissertation research, I used chemical tools to identify Mtb enzymatic activity associated with dormant, reactivating, and active Mtb. My research focused on esterases, including the lipase subclass, which influence Mtb pathogenicity and reactivation from dormancy. 000003012 542__ $$fIn copyright - single owner 000003012 650__ $$aVirulence$$027844 000003012 650__ $$aLipase$$021533 000003012 650__ $$aEsterases$$018648 000003012 650__ $$aImmune System$$020666 000003012 650__ $$aLatent Tuberculosis$$038534 000003012 650__ $$aMycobacterium tuberculosis$$022570 000003012 650__ $$aLung Diseases$$021647 000003012 650__ $$aTuberculosis$$027470 000003012 691__ $$aSchool of Medicine$$041369 000003012 692__ $$aDepartment of Physiology and Pharmacology$$041442 000003012 7001_ $$aTallman, Katie$$uOregon Health and Science University$$041354$$10000-0002-9643-6102 000003012 7201_ $$aBeatty, Kimberly$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor 000003012 7201_ $$aScanlan, Thomas$$uOregon Health and Science University$$041354$$7Personal$$eCommittee chair 000003012 7201_ $$aUllman, Buddy$$uOregon Health and Science University$$041354$$7Personal$$eCommittee member 000003012 7201_ $$aCohen, Michael$$uOregon Health and Science University$$041354$$7Personal$$eCommittee member 000003012 7201_ $$aGibbs, Summer$$uOregon Health and Science University$$041354$$7Personal$$eCommittee member 000003012 7201_ $$aTafesse, Fikadu$$uOregon Health and Science University$$041354$$7Personal$$eCommittee member 000003012 8564_ $$90e8ba57d-afea-45b8-96bb-9271445c7509$$s14997492$$uhttps://digitalcollections.ohsu.edu/record/3012/files/3815_etd.pdf$$ePublic$$2f183b485ddca96242d588e04a205fbc6$$31 000003012 905__ $$a/rest/prod/nc/58/0m/93/nc580m93r 000003012 909CO $$ooai:digitalcollections.ohsu.edu:3012$$pstudent-work 000003012 980__ $$aTheses and Dissertations