TY  - GEN
AB  - LAVA (LINE-Alu-VNTR-Alu-like) elements are non-autonomous retrotransposons specific to gibbons and may have influenced their evolution. These elements contain segments from various primate repeats. We tested if a full-length LAVA element, LAVAC, can be mobilized by human LINE-1 proteins in HeLa cells. Results show L1 proteins mobilize LAVAC over 100 times more than processed pseudogenes and three times more than human SVAE retrotransposition. Only the SVA-derived portions are active, and truncating the 3’ L1ME5 part boosts retrotransposition rates by at least 100%. Tagged insertions matched findings in the gibbon genome, suggesting new models for the LAVA retrotransposon's evolution.
AD  - Oregon Health and Science University
AU  - Meyer, Thomas
DA  - 2016-12-01
DO  - 10.6083/M4H41QG6
DO  - DOI
ED  - Carbone, Lucia
ED  - Advisor
ID  - 3034
KW  - Long Interspersed Nucleotide Elements
KW  - Retroelements
KW  - HeLa Cells
KW  - Primates
KW  - Hylobates
L1  - https://digitalcollections.ohsu.edu/record/3034/files/3859_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/3034/files/3859_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/3034/files/3859_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/3034/files/3859_etd.pdf
N2  - LAVA (LINE-Alu-VNTR-Alu-like) elements are non-autonomous retrotransposons specific to gibbons and may have influenced their evolution. These elements contain segments from various primate repeats. We tested if a full-length LAVA element, LAVAC, can be mobilized by human LINE-1 proteins in HeLa cells. Results show L1 proteins mobilize LAVAC over 100 times more than processed pseudogenes and three times more than human SVAE retrotransposition. Only the SVA-derived portions are active, and truncating the 3’ L1ME5 part boosts retrotransposition rates by at least 100%. Tagged insertions matched findings in the gibbon genome, suggesting new models for the LAVA retrotransposon's evolution.
PY  - 2016-12-01
T1  - The flow of the Gibbon LAVA Element is facilitated by the LINE-1 retrotransposition machinery
TI  - The flow of the Gibbon LAVA Element is facilitated by the LINE-1 retrotransposition machinery
UR  - https://digitalcollections.ohsu.edu/record/3034/files/3859_etd.pdf
Y1  - 2016-12-01
ER  -