000003135 001__ 3135 000003135 005__ 20251013145334.0 000003135 0247_ $$2DOI$$a10.6083/Z4H07L 000003135 037__ $$aETD 000003135 245__ $$aDevelopment of tissue-specific fluorescent contrast agents and direct application methods for improved clinical translation of fluorescence-guided surgery 000003135 260__ $$bOregon Health and Science University 000003135 269__ $$a2018 000003135 336__ $$aThesis 000003135 502__ $$bM.S. 000003135 520__ $$aOver 300 million surgeries are performed worldwide each year. The ultimate goal of surgery is to repair damage or remove diseased tissues, while minimizing comorbidities by preserving vital structures such as nerves and blood vessels. However, surgeons still rely mainly on the basic tools of naked eye visualization and palpation for guidance during surgery, leaving incomplete resection rates high and comorbidities like nerve damage as a major problem. Fluorescence-guided surgery (FGS) has the potential to revolutionize surgery by enhancing visualization of specific tissues intraoperatively. Using optical imaging of targeted fluorescent probes, FGS offers sensitive, real-time, wide-field imaging using compact imaging systems that are easily integrated into the operating room. Several imaging systems are clinically available for FGS applications, however only a handful of contrast agents have been approved. Numerous preclinical studies for new agents have shown promise for improved surgical outcomes such as tumor resection or nerve-sparing. However, as non-curative diagnostic agents these new probes must overcome an enormous regulatory challenge to achieve clinical translation. The work presented herein has been carried out to develop novel targeted contrast agents for tumor margin detection and nerve identification as well as create more clinically viable administration methods to aid in their clinical translation. 000003135 540__ $$fCC BY 000003135 542__ $$fIn copyright - single owner 000003135 650__ $$aOptical Imaging$$039980 000003135 650__ $$aFluorescence$$019121 000003135 650__ $$aOptical Imaging$$039980 000003135 6531_ $$aNerve sparing 000003135 6531_ $$aimage guided surgery 000003135 691__ $$aSchool of Medicine$$041369 000003135 7001_ $$aBarth, Connor$$uOregon Health and Science University$$041354$$10000-0002-6235-7238 000003135 7201_ $$aGibbs, Summer$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor$$eMentor 000003135 8564_ $$923026eb0-8bad-4903-a1de-6ba2f6c2f4a3$$s34671460$$uhttps://digitalcollections.ohsu.edu/record/3135/files/4099_etd.pdf$$ePublic$$2fd4c67054cf89065007f5db3854a58f9$$31 000003135 905__ $$a/rest/prod/zg/64/tm/15/zg64tm15k 000003135 909CO $$ooai:digitalcollections.ohsu.edu:3135$$pstudent-work 000003135 980__ $$aTheses and Dissertations