000000340 001__ 340
000000340 005__ 20260303112335.0
000000340 0247_ $$2DOI$$a10.6083/M4XG9P32
000000340 037__ $$aETD
000000340 245__ $$aRole of cytoskeletal alignment, independent of fluid shear stress, in endothelial cell functions
000000340 260__ $$bOregon Health and Science University
000000340 269__ $$a2009
000000340 336__ $$aDissertation
000000340 502__ $$bPh.D.
000000340 502__ $$gBiomedical Engineering
000000340 520__ $$aAtherosclerosis is closely linked to endothelial cell (EC) function, which is strongly influenced by fluid shear stress (FSS). While high, unidirectional FSS produces elongated, aligned ECs with athero‑protective behaviors, low or oscillatory FSS results in cobblestone‑shaped ECs that promote an athero‑prone environment. Whether EC shape itself, independent of FSS, regulates EC function is not well understood. Using micropatterned protein lanes to induce EC elongation without flow, this study shows that elongated EC shape and cytoskeletal alignment reproduce several protective functional characteristics, including extracellular matrix deposition, reduced inflammation, and lower thrombotic potential. These findings suggest that cytoskeletal organization actively contributes to EC functions that support cardiovascular health, independent of mechanical shear forces.
000000340 540__ $$fCC BY
000000340 542__ $$fIn copyright - single owner
000000340 650__ $$aCytoskeleton$$017362
000000340 650__ $$aAtherosclerosis$$036329
000000340 650__ $$aCardiovascular Diseases$$016152
000000340 6531_ $$afluid shear stress
000000340 6531_ $$aendothelial cell
000000340 6531_ $$avascular endothelium
000000340 6531_ $$amicropattern
000000340 691__ $$aSchool of Medicine$$041369
000000340 692__ $$aDepartment of Science and Engineering$$041447
000000340 7001_ $$aVartanian, Keri$$uOregon Health and Science University$$041354
000000340 7201_ $$aHinds, Monica$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000340 8564_ $$99ddcceed-9603-4176-af0e-e0c9e14ca32b$$s9604648$$uhttps://digitalcollections.ohsu.edu/record/340/files/341_etd.pdf$$ePublic$$2069a8600cb51c37f458b128f2f0d9258$$31
000000340 905__ $$a/rest/prod/pv/63/g0/24/pv63g024f
000000340 909CO $$ooai:digitalcollections.ohsu.edu:340$$pstudent-work
000000340 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000340 980__ $$aTheses and Dissertations