000000346 001__ 346
000000346 005__ 20260306112717.0
000000346 0247_ $$2DOI$$a10.6083/M4513W4D
000000346 037__ $$aETD
000000346 245__ $$aRegulation of the transcription factor p63 by βTrCP
000000346 260__ $$bOregon Health and Science University
000000346 269__ $$a2008
000000346 336__ $$aDissertation
000000346 502__ $$bPh.D.
000000346 520__ $$aThis study identifies SCFβTrCP1 as an E3 ubiquitin ligase that selectively stabilizes and activates the TAp63γ isoform. βTrCP1 increases TAp63γ protein levels and half-life through direct binding and K48‑linked ubiquitylation at the N‑terminus. This stabilization enhances TAp63γ‑driven p21 transcription and promotes G1/S cell‑cycle arrest. A ligase‑deficient βTrCP1 mutant blocks these effects, confirming ubiquitylation is required. Overall, these findings reveal a mechanism by which SCFβTrCP1 differentially regulates p63 isoforms, advancing understanding of p63’s role in epithelial homeostasis and cancer.
000000346 540__ $$fCC BY
000000346 542__ $$fIn copyright - single owner
000000346 650__ $$aCell Cycle$$016278
000000346 650__ $$aUbiquitination$$038090
000000346 650__ $$aSKP Cullin F-Box Protein Ligases$$035541
000000346 650__ $$aTumor Suppressor Protein p53$$028922
000000346 650__ $$aProto-Oncogene Proteins p21(ras)$$029033
000000346 650__ $$aProtein Isoforms$$032074
000000346 650__ $$aS Phase$$028956
000000346 6531_ $$ap63
000000346 6531_ $$aactivation
000000346 6531_ $$ap53 family
000000346 6531_ $$ap21
000000346 6531_ $$ascf
000000346 6531_ $$abetatrcp
000000346 691__ $$aSchool of Medicine$$041369
000000346 692__ $$aDepartment of Biochemistry and Molecular Biology$$041396
000000346 7001_ $$aGallegos, Jayme$$uOregon Health and Science University$$041354
000000346 7201_ $$aLu, Hua$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000346 8564_ $$9e3426ed5-fe8f-4e12-9af8-9cb291d46465$$s2099225$$uhttps://digitalcollections.ohsu.edu/record/346/files/347_etd.pdf$$ePublic$$2937e6bc72084397fc1127abc1bfdecfd$$31
000000346 905__ $$a/rest/prod/ms/35/t8/62/ms35t8620
000000346 909CO $$ooai:digitalcollections.ohsu.edu:346$$pstudent-work
000000346 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000346 980__ $$aTheses and Dissertations