TY  - GEN
AB  - This thesis presents two advances for improving gene expression in Pichia pastoris: a more efficient method for selecting multicopy transformants and a new endogenous selectable marker. A posttransformational vector amplification (PTVA) strategy was developed, enabling low‑copy transformants to increase vector copy number and yielding stable tandem genomic integrations. In addition, the FLD1 gene was established as an effective selectable marker in an fld1 mutant strain, offering efficient transformation without exogenous bacterial sequences and supporting copy‑number enhancement. Together, these tools provide valuable alternatives for achieving high‑yield protein expression in P. pastoris.
AD  - Oregon Health and Science University
AU  - Sunga, Anthony
DA  - 2008
DO  - 10.6083/M41834FH
DO  - DOI
ED  - Cregg, James
ED  - Advisor
ID  - 347
KW  - Gene Expression
KW  - Saccharomyces cerevisiae
KW  - DNA Copy Number Variations
KW  - Biomarkers
KW  - Komagataella pastoris
KW  - pichia pastoris
KW  - pichia postoris multicopy selection selectable markers pichia postoris
KW  - biological markers
KW  - selectable markers
KW  - multicopy selection
KW  - biochemical markers
L1  - https://digitalcollections.ohsu.edu/record/347/files/348_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/347/files/348_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/347/files/348_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/347/files/348_etd.pdf
N2  - This thesis presents two advances for improving gene expression in Pichia pastoris: a more efficient method for selecting multicopy transformants and a new endogenous selectable marker. A posttransformational vector amplification (PTVA) strategy was developed, enabling low‑copy transformants to increase vector copy number and yielding stable tandem genomic integrations. In addition, the FLD1 gene was established as an effective selectable marker in an fld1 mutant strain, offering efficient transformation without exogenous bacterial sequences and supporting copy‑number enhancement. Together, these tools provide valuable alternatives for achieving high‑yield protein expression in P. pastoris.
PB  - Oregon Health and Science University
PY  - 2008
T1  - Novel methods for generating strains with increased copy number in the yeast Pichia pastoris
TI  - Novel methods for generating strains with increased copy number in the yeast Pichia pastoris
UR  - https://digitalcollections.ohsu.edu/record/347/files/348_etd.pdf
Y1  - 2008
ER  -