000000348 001__ 348
000000348 005__ 20260410124446.0
000000348 0247_ $$2DOI$$a10.6083/M4WH2MZT
000000348 037__ $$aETD
000000348 245__ $$aCharacterization of mutant carnitine palmitoyltransferase I gene expressed in yeast and mice
000000348 260__ $$bOregon Health and Science University
000000348 269__ $$a2008
000000348 336__ $$aDissertation
000000348 502__ $$bPh.D.
000000348 520__ $$aCarnitine palmitoyltransferase I (CPTI) is the rate‑limiting enzyme in mitochondrial fatty acid β‑oxidation and is regulated by malonyl‑CoA. This dissertation investigates structural, regulatory, and physiological roles of CPTI using molecular and mouse models. Mutational analysis of the N‑ and C‑terminal domains of liver CPTI demonstrates compensatory and additive effects on malonyl‑CoA sensitivity, supporting interdomain regulatory interactions. In vivo studies using muscle CPTI knockout and transgenic mouse models reveal that CPTI is essential for development, regulates fatty acid metabolism during fasting, and influences body weight, lipid storage, and susceptibility to diabetes. These findings highlight CPTI as a key regulator of energy metabolism and a potential therapeutic target for metabolic disease.
000000348 540__ $$fCC BY
000000348 542__ $$fIn copyright - single owner
000000348 650__ $$aFasting$$018901
000000348 650__ $$aBody Weight$$015708
000000348 650__ $$aMalonyl Coenzyme A$$021779
000000348 650__ $$aMice$$036842
000000348 650__ $$aMice, Transgenic$$022253
000000348 650__ $$aMetabolic Diseases$$022100
000000348 650__ $$aCarnitine O-Palmitoyltransferase$$016167
000000348 650__ $$aDiabetes Mellitus$$017671
000000348 650__ $$aDiet, High-Fat$$039538
000000348 6531_ $$aglobal gene knockout
000000348 6531_ $$ain vivo overexpression
000000348 6531_ $$amutation activity
000000348 6531_ $$amuscle isoform
000000348 691__ $$aSchool of Medicine$$041369
000000348 692__ $$aDepartment of Science and Engineering$$041447
000000348 7001_ $$aChen, Zhan$$uOregon Health and Science University$$041354
000000348 7201_ $$aWoldegiorgis, Gebretateos$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000348 8564_ $$90a99ae73-d9d4-4b33-8742-a02a79cee2d3$$s984436$$uhttps://digitalcollections.ohsu.edu/record/348/files/349_etd.pdf$$ePublic$$282e3fd1ff0110395d808620f250ee108$$31
000000348 901__ $$a<p>These documents are archival records. They are retained for historical reference only. </p><p><b>Need an accessible version? Use the ‘Get Accessible Copy’ link above.</b></p>
000000348 905__ $$a/rest/prod/9z/90/2z/85/9z902z85m
000000348 909CO $$ooai:digitalcollections.ohsu.edu:348$$pstudent-work
000000348 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000348 980__ $$aTheses and Dissertations