000000361 001__ 361
000000361 005__ 20260302091151.0
000000361 0247_ $$2DOI$$a10.6083/M46T0JMN
000000361 037__ $$aETD
000000361 245__ $$aDiffusion-based single particle sizing method provides precise measurement of nanoparticle individual size variation and formation of bioconjugates
000000361 260__ $$bOregon Health and Science University
000000361 269__ $$a2009
000000361 336__ $$aThesis
000000361 502__ $$bM.S.
000000361 520__ $$aWe present diffusion‑based single particle sizing (DBSPS), a method for determining hydrodynamic size distributions of fluorescent quantum dots (QDs) and QD bioconjugates. Using fluorescence video microscopy, individual particle trajectories were recorded and analyzed via mean square displacement to calculate diffusion coefficients, which were then converted to hydrodynamic radii using the Stokes–Einstein equation. Unlike ensemble measurements, DBSPS characterizes single particles within heterogeneous samples, enabling detection of QD–biomolecule conjugation efficiency and exclusion of aggregated QD complexes. Results confirm that DBSPS accurately measures hydrodynamic dimensions of QDs and nanospheres, offering a powerful tool for optimizing QD bioconjugate design in aqueous environments.
000000361 540__ $$fCC BY
000000361 542__ $$fIn copyright - single owner
000000361 650__ $$aMethods$$022159
000000361 650__ $$aMolecular Biology$$022384
000000361 650__ $$aMicroscopy$$022282
000000361 650__ $$aQuantum Dots$$035681
000000361 650__ $$aStaining and Labeling$$026352
000000361 650__ $$aFluorescent Dyes$$019124
000000361 650__ $$aHydrodynamics$$038932
000000361 650__ $$aSingle Molecule Imaging$$012091
000000361 6531_ $$abioconjugate particle tracking
000000361 6531_ $$ahydrodynamic size
000000361 691__ $$aSchool of Medicine$$041369
000000361 692__ $$aDepartment of Science and Engineering$$041447
000000361 7001_ $$aArdeshiri, Ardalan$$uOregon Health and Science University$$041354
000000361 7201_ $$aVu, Tania$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000361 8564_ $$9528e11e2-4c5f-4a61-b14c-aa45d1e6dafb$$s1040912$$uhttps://digitalcollections.ohsu.edu/record/361/files/362_etd.pdf$$ePublic$$25414629ff4c431c2014a0bdcb9de0f17$$31
000000361 905__ $$a/rest/prod/v9/79/v3/06/v979v3061
000000361 909CO $$ooai:digitalcollections.ohsu.edu:361$$pstudent-work
000000361 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000361 980__ $$aTheses and Dissertations