000000362 001__ 362
000000362 005__ 20260218081040.0
000000362 0247_ $$2DOI$$a10.6083/M4348H9D
000000362 037__ $$aETD
000000362 245__ $$aGenetic analysis reveals complex Mn(II) oxidation regulation in Pseudomonas putida GB-1
000000362 260__ $$bOregon Health and Science University
000000362 269__ $$a2010
000000362 336__ $$aThesis
000000362 502__ $$bM.S.
000000362 520__ $$aManganese is a widely abundant transition metal, yet the biochemical mechanisms behind bacterial Mn(II) oxidation remain poorly defined. To identify genes involved in this process, random transposon mutagenesis was performed in Pseudomonas putida GB‑1. Mutants with reduced Mn(II) oxidation were identified, but unexpectedly, several mutants oxidized Mn(II) earlier than the wild‑type. All of these carried disruptions in motility‑related genes, and further assays confirmed a link between impaired motility or flagella synthesis and accelerated Mn(II) oxidation—the first evidence of such a relationship. Growth‑condition experiments also showed that different genes are required for Mn(II) oxidation depending on substrate and temperature. Together, these results reveal the complexity of Mn(II) oxidation regulation in P. putida GB‑1.
000000362 540__ $$fCC BY
000000362 542__ $$fIn copyright - single owner
000000362 650__ $$aPseudomonas putida$$029564
000000362 650__ $$aFlagella$$019078
000000362 650__ $$aManganese$$021806
000000362 650__ $$aOxidation-Reduction$$023418
000000362 650__ $$aBiofilms$$030801
000000362 650__ $$aPseudomonas$$024802
000000362 6531_ $$aoxidation-reduation reaction
000000362 6531_ $$apseudomonas putida gb-1
000000362 691__ $$aSchool of Medicine$$041369
000000362 692__ $$aDepartment of Science and Engineering$$041447
000000362 7001_ $$aYamaguchi, Ai$$uOregon Health and Science University$$041354
000000362 7201_ $$aTebo, Bradley$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000362 8564_ $$93bb40a86-d925-4e81-8ea3-5394af02f725$$s2829414$$uhttps://digitalcollections.ohsu.edu/record/362/files/363_etd.pdf$$ePublic$$292d4813c0671e5cc92360be4bc283833$$31
000000362 905__ $$a/rest/prod/vq/27/zn/42/vq27zn42r
000000362 909CO $$ooai:digitalcollections.ohsu.edu:362$$pstudent-work
000000362 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000362 980__ $$aTheses and Dissertations