TY  - GEN
AB  - This thesis demonstrates how optical coherence tomography (OCT) can be used to quantitatively characterize engineered collagen gels containing smooth muscle cells. By fitting OCT depth‑dependent signals to a theoretical model, the scattering coefficient (μs) and anisotropy factor (g) were extracted to assess changes in scattering particle density and size. Over five days, collagen gels exhibited a ten‑fold increase in reflectivity without changes in attenuation, indicating decreased anisotropy and increased backscatter. Follow‑up experiments showed that matrix metalloproteinase (MMP) activity from embedded smooth muscle cells degraded collagen fibrils into smaller, more isotropic scatterers. These results demonstrate that OCT can noninvasively visualize and quantify MMP‑driven matrix remodeling in collagen gels.
AD  - Oregon Health and Science University
AU  - Levitz, David
DA  - 2010
DO  - 10.6083/M4J38QGT
DO  - DOI
ED  - Jacques, Steven
ED  - Advisor
ID  - 374
KW  - Colloids
KW  - Tomography
KW  - Matrix Metalloproteinases
KW  - Tissue Engineering
KW  - Gels
KW  - Collagen
KW  - Tomography, Optical Coherence
KW  - anisotrophy
KW  - matrix remodeling
L1  - https://digitalcollections.ohsu.edu/record/374/files/375_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/374/files/375_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/374/files/375_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/374/files/375_etd.pdf
N2  - This thesis demonstrates how optical coherence tomography (OCT) can be used to quantitatively characterize engineered collagen gels containing smooth muscle cells. By fitting OCT depth‑dependent signals to a theoretical model, the scattering coefficient (μs) and anisotropy factor (g) were extracted to assess changes in scattering particle density and size. Over five days, collagen gels exhibited a ten‑fold increase in reflectivity without changes in attenuation, indicating decreased anisotropy and increased backscatter. Follow‑up experiments showed that matrix metalloproteinase (MMP) activity from embedded smooth muscle cells degraded collagen fibrils into smaller, more isotropic scatterers. These results demonstrate that OCT can noninvasively visualize and quantify MMP‑driven matrix remodeling in collagen gels.
PB  - Oregon Health and Science University
PY  - 2010
T1  - Measuring optical properties of engineered tissues using optical coherence tomography
TI  - Measuring optical properties of engineered tissues using optical coherence tomography
UR  - https://digitalcollections.ohsu.edu/record/374/files/375_etd.pdf
Y1  - 2010
ER  -