000000374 001__ 374
000000374 005__ 20260219134606.0
000000374 0247_ $$2DOI$$a10.6083/M4J38QGT
000000374 037__ $$aETD
000000374 245__ $$aMeasuring optical properties of engineered tissues using optical coherence tomography
000000374 260__ $$bOregon Health and Science University
000000374 269__ $$a2010
000000374 336__ $$aDissertation
000000374 502__ $$bPh.D.
000000374 520__ $$aThis thesis demonstrates how optical coherence tomography (OCT) can be used to quantitatively characterize engineered collagen gels containing smooth muscle cells. By fitting OCT depth‑dependent signals to a theoretical model, the scattering coefficient (μs) and anisotropy factor (g) were extracted to assess changes in scattering particle density and size. Over five days, collagen gels exhibited a ten‑fold increase in reflectivity without changes in attenuation, indicating decreased anisotropy and increased backscatter. Follow‑up experiments showed that matrix metalloproteinase (MMP) activity from embedded smooth muscle cells degraded collagen fibrils into smaller, more isotropic scatterers. These results demonstrate that OCT can noninvasively visualize and quantify MMP‑driven matrix remodeling in collagen gels.
000000374 540__ $$fCC BY
000000374 542__ $$fIn copyright - single owner
000000374 650__ $$aColloids$$016898
000000374 650__ $$aTomography$$027155
000000374 650__ $$aMatrix Metalloproteinases$$032691
000000374 650__ $$aTissue Engineering$$033051
000000374 650__ $$aGels$$019433
000000374 650__ $$aCollagen$$016890
000000374 650__ $$aTomography, Optical Coherence$$035114
000000374 6531_ $$aanisotrophy
000000374 6531_ $$amatrix remodeling
000000374 691__ $$aSchool of Medicine$$041369
000000374 692__ $$aDepartment of Biomedical Engineering$$041397
000000374 7001_ $$aLevitz, David$$uOregon Health and Science University$$041354
000000374 7201_ $$aJacques, Steven$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000374 8564_ $$9a3fc210a-4a79-4429-9386-09117df53296$$s19715745$$uhttps://digitalcollections.ohsu.edu/record/374/files/375_etd.pdf$$ePublic$$2502556ef538f6f75c5e4b48f06e495da$$31
000000374 905__ $$a/rest/prod/69/69/z0/77/6969z0774
000000374 909CO $$ooai:digitalcollections.ohsu.edu:374$$pstudent-work
000000374 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000374 980__ $$aTheses and Dissertations