000000042 001__ 42
000000042 005__ 20260428125922.0
000000042 0247_ $$2DOI$$a10.6083/M4JS9NCX
000000042 037__ $$aETD
000000042 245__ $$aSpecific requirements for translational regulation by a nascent peptide that stalls ribosomes in response to arginine
000000042 260__ $$bOregon Health and Science University
000000042 269__ $$a2006
000000042 336__ $$aDissertation
000000042 502__ $$bPh.D.
000000042 520__ $$aThe Neurospora crassa arg‑2 gene encodes a key enzyme in arginine biosynthesis and is regulated translationally by an upstream open reading frame encoding the arginine attenuator peptide (AAP). In response to high arginine levels, synthesis of the evolutionarily conserved AAP causes ribosome stalling, thereby reducing downstream translation. This study demonstrates that AAP function depends on its amino acid sequence rather than its nucleotide sequence and that it can regulate translation when positioned either upstream or within a larger polypeptide. Mutational and deletion analyses identified a minimal conserved domain required for arginine‑specific regulation. Together, these findings establish the AAP as a conserved peptide domain capable of controlling translational elongation in response to metabolite levels.
000000042 540__ $$fCC BY
000000042 542__ $$fIn copyright - single owner
000000042 650__ $$aRibosomes$$025490
000000042 650__ $$aNeurospora crassa$$022874
000000042 650__ $$aarginine attenuator peptide
000000042 691__ $$aOGI School of Science and Engineering$$041365
000000042 692__ $$aDepartment of Environmental and Biomolecular Systems$$041414
000000042 7001_ $$aSpevak, Christina$$uOregon Health and Science University$$041354
000000042 7201_ $$aSachs, Matthew$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000042 8564_ $$902ac940c-f828-43ef-b6b7-34a6a970ec68$$s2137551$$uhttps://digitalcollections.ohsu.edu/record/42/files/42_etd.pdf$$ePublic$$28fdeb94450c40451a606064ede8885c1$$31
000000042 901__ $$a&lt;p&gt;These documents are archival records. They are retained for historical reference only. &lt;/p&gt;&lt;p&gt;&lt;b&gt;Need an accessible version? Use the ‘Get Accessible Copy’ link above.&lt;/b&gt;&lt;/p&gt;
000000042 905__ $$a/rest/prod/fq/97/7t/76/fq977t769
000000042 909CO $$ooai:digitalcollections.ohsu.edu:42$$pstudent-work
000000042 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000042 980__ $$aTheses and Dissertations