@article{ETD,
      school = {Ph.D.},
      author = {Shumate, Anthony},
      url = {http://digitalcollections.ohsu.edu/record/42008},
      title = {Gα subunit C-terminal interactions with GPCRs: novel  analysis and applications},
      publisher = {Oregon Health and Science University},
      abstract = {G protein-coupled receptors (GPCRs) play an essential role  in signal transduction by transmitting extracellular  stimuli into intracellular responses through activation of  G proteins.  The binding interaction between GPCRs and the  Gα subunit of G proteins is a fundamental step in this  process, initiating a cascade of downstream events.   Interestingly, there are >800 members of the GPCR  superfamily yet only 5 main classes of Gα subunit are  needed to maintain homeostasis.  How then, can this small  set of Gα proteins bind to and recognize such a diverse  family of receptors?   The main goals of my thesis are: 1)  to investigate this conserved interaction, primarily  through studies of the visual GPCR rhodopsin and the  high-affinity Gα-CT peptide, 2) to apply the insights  gained to design novel tools for examining conformational  equilibrium at rhodopsin (and other GPCRs) using  biophysical and biochemical approaches, 3) to leverage our  understanding of this key interaction to develop new ways  to purify and study these vital receptors.},
      number = {ETD},
      doi = {https://doi.org/10.6083/bpxhc42008},
      recid = {42008},
      address = {2023-09-22},
}