000042008 001__ 42008 000042008 005__ 20240124114340.0 000042008 0247_ $$2doi$$a10.6083/bpxhc42008 000042008 037__ $$aETD 000042008 041__ $$aeng 000042008 245__ $$aGα subunit C-terminal interactions with GPCRs: novel analysis and applications 000042008 260__ $$bOregon Health and Science University 000042008 269__ $$a2023-09-22 000042008 336__ $$aDissertation 000042008 502__ $$bPh.D. 000042008 502__ $$gBiochemistry & Molecular Biology 000042008 520__ $$aG protein-coupled receptors (GPCRs) play an essential role in signal transduction by transmitting extracellular stimuli into intracellular responses through activation of G proteins. The binding interaction between GPCRs and the Gα subunit of G proteins is a fundamental step in this process, initiating a cascade of downstream events. Interestingly, there are >800 members of the GPCR superfamily yet only 5 main classes of Gα subunit are needed to maintain homeostasis. How then, can this small set of Gα proteins bind to and recognize such a diverse family of receptors? The main goals of my thesis are: 1) to investigate this conserved interaction, primarily through studies of the visual GPCR rhodopsin and the high-affinity Gα-CT peptide, 2) to apply the insights gained to design novel tools for examining conformational equilibrium at rhodopsin (and other GPCRs) using biophysical and biochemical approaches, 3) to leverage our understanding of this key interaction to develop new ways to purify and study these vital receptors. 000042008 540__ $$fCC BY 000042008 542__ $$fIn copyright - single owner 000042008 650__ $$aReceptors, G-Protein-Coupled$$035314 000042008 650__ $$aRhodopsin$$025463 000042008 650__ $$aMembrane Proteins$$022009 000042008 650__ $$aFluorescence$$019121 000042008 650__ $$aChromatography, Affinity$$016654 000042008 6531_ $$aprotein purification 000042008 691__ $$aSchool of Medicine$$041369 000042008 692__ $$aDepartment of Biochemistry and Molecular Biology$$041396 000042008 7001_ $$aShumate, Anthony$$uOregon Health and Science University$$041354 000042008 7201_ $$aFarrens, David$$uOregon Health and Science University$$041354$$eAdvisor$$7Personal 000042008 8564_ $$99f2edeca-4b67-4774-b24a-6e6bc81f451a$$s8756597$$uhttps://digitalcollections.ohsu.edu/record/42008/files/Shumate.Anthony.2023.pdf 000042008 909CO $$ooai:digitalcollections.ohsu.edu:42008$$pstudent-work 000042008 980__ $$aTheses and Dissertations 000042008 981__ $$aPublished$$b2023-09-22