TY  - GEN
N2  - Poly-(ADP)-ribose polymerases (PARPs) are a family 17 enzymes in humans that have diverse roles in cell biology including innate immune response, DNA repair, and regulation of signaling pathways. PARPs catalyze the transfer of ADP-ribose from nicotinamide adenine dinucleotide (NAD+) to target proteins or biomolecules. This enigmatic post- translational modification comes in two varieties: the transfer of a single unit of ADP-ribose, known as mono-ADP-ribosylation (MARylation) or the transfer of multiple units of ADP-ribose, known as poly-ADP-ribosylation (PARylation). The overarching goal of this work was to leverage chemical tools to further the field’s understanding of the biological roles and regulation of PARPs. The work can be divided into two distinct parts: 1) the development of tools for studying cellular mechanisms and 2) the application of small-molecule tools to uncover previously unknown relationships between writers and erasers of MARylation.
DO  - 10.6083/bpxhc42299
DO  - doi
AB  - Poly-(ADP)-ribose polymerases (PARPs) are a family 17 enzymes in humans that have diverse roles in cell biology including innate immune response, DNA repair, and regulation of signaling pathways. PARPs catalyze the transfer of ADP-ribose from nicotinamide adenine dinucleotide (NAD+) to target proteins or biomolecules. This enigmatic post- translational modification comes in two varieties: the transfer of a single unit of ADP-ribose, known as mono-ADP-ribosylation (MARylation) or the transfer of multiple units of ADP-ribose, known as poly-ADP-ribosylation (PARylation). The overarching goal of this work was to leverage chemical tools to further the field’s understanding of the biological roles and regulation of PARPs. The work can be divided into two distinct parts: 1) the development of tools for studying cellular mechanisms and 2) the application of small-molecule tools to uncover previously unknown relationships between writers and erasers of MARylation.
T1  - Leveraging small molecule tools to study protein ADP-ribosylation
ED  - Cohen, Michael
ED  - PI
DA  - 2023-10-31
AU  - Sanderson, Daniel J
L1  - https://digitalcollections.ohsu.edu/record/42299/files/Sanderson.Daniel.2023.pdf
PB  - Oregon Health and Science University
LA  - eng
PY  - 2023-10-31
ID  - 42299
L4  - https://digitalcollections.ohsu.edu/record/42299/files/Sanderson.Daniel.2023.pdf
KW  - Poly(ADP-ribose) Polymerases
KW  - ADP Ribose Transferases
KW  - Photoaffinity Labels
KW  - Poly(ADP-ribose) Polymerase Inhibitors
KW  - parps
KW  - chemical tools
KW  - adp-ribosylation
KW  - marylation
KW  - parg
TI  - Leveraging small molecule tools to study protein ADP-ribosylation
Y1  - 2023-10-31
L2  - https://digitalcollections.ohsu.edu/record/42299/files/Sanderson.Daniel.2023.pdf
LK  - https://digitalcollections.ohsu.edu/record/42299/files/Sanderson.Daniel.2023.pdf
UR  - https://digitalcollections.ohsu.edu/record/42299/files/Sanderson.Daniel.2023.pdf
ER  -