@article{ETD,
      school = {Ph.D.},
      author = {Fallon, Meghan},
      url = {http://digitalcollections.ohsu.edu/record/42402},
      title = {The effects of topographical micropatterning on acute  thrombosis and endothelial cell (patho)physiology},
      publisher = {Oregon Health and Science University},
      abstract = {Cardiovascular disease affects nearly half of all U.S.  adults and is the leading cause of death worldwide.  Advanced cases are often treated through vascular grafting  to bypass occluded vessels. Synthetic vascular graft  materials suffer from patency complications due to  thrombosis and neointimal growth impeding the materials’  long-term function for small-diameter applications. Thus,  there is a critical unmet need for improved biocompatible  small-diameter vascular grafts to support long-term patient  outcomes and reduce re-intervention procedures. The in  vitro establishment of an endothelial layer on synthetic  vascular grafts has been suggested to be a solution due to  the endothelial cells’ (ECs) homeostatic capabilities to  prevent thrombus formation and limit immunogenicity.  Therefore, vascular graft material surfaces which limit  thrombosis and support EC growth and function are a  significant clinical need. A surface modification that has  the potential to attenuate thrombosis while promoting an  endothelium is topographical micropatterning on luminal  biomaterial surfaces. Topographical micropatterning is a  physical modification that downregulates platelet adhesion  and activation in static culture while promoting  endothelial migration and function. This work provides a  systematic study of the in vitro thrombogenicity of  micropatterned hydrogel surfaces with varying feature sizes  as well as ex vivo assessments of acute thrombogenesis on  the modified grafts. Additionally, this work utilizes  topographical micropatterns as a platform to induce  endothelial elongation and cytoskeletal alignment to  investigate changes in mechanotransduction pathways  independent of blood fluid shear stress. The culmination of  these findings aids in the design of novel small-diameter  synthetic vascular grafts and provide insight into the  mechanisms by which elongated ECs transduce an  anti-inflammatory phenotype.},
      number = {ETD},
      doi = {https://doi.org/10.6083/bpxhc42402},
      recid = {42402},
      address = {2023-11-30},
}