000000430 001__ 430 000000430 005__ 20260224132750.0 000000430 0247_ $$2DOI$$a10.6083/M498850G 000000430 037__ $$aETD 000000430 245__ $$aSalmonella characterization of the type III effector SrfH and proteomic identification of the secretome 000000430 260__ $$bOregon Health and Science University 000000430 269__ $$a2010 000000430 336__ $$aDissertation 000000430 502__ $$bPh.D. 000000430 520__ $$aSalmonella is a major global pathogen, causing millions of infections and significant mortality, particularly among HIV‑infected individuals in sub‑Saharan Africa. Its virulence depends on effector proteins delivered into host cells by two type III secretion systems (SPI‑1 and SPI‑2). This work shows that the SPI‑2 effector SrfH drives rapid septicemia by interacting with the host protein TRIP6, likely through a c‑Src–dependent pathway. To expand the known effector repertoire, I used two strategies: a transposon‑based reporter system and LC‑MS/MS analysis of proteins secreted under SPI‑2–inducing conditions. Mass spectrometry identified over 200 secreted proteins, including numerous type III substrates and several effectors secreted independently of type III systems. These findings reveal new aspects of Salmonella pathogenesis and highlight additional secretion pathways that warrant future study. 000000430 540__ $$fCC BY 000000430 542__ $$fIn copyright - single owner 000000430 650__ $$aSalmonella$$025680 000000430 650__ $$aVirulence Factors$$034836 000000430 650__ $$aBacterial Proteins$$015330 000000430 650__ $$aGenomic Islands$$035469 000000430 650__ $$aSepsis$$031109 000000430 650__ $$aAfrica South of the Sahara 000000430 691__ $$aSchool of Medicine$$041369 000000430 692__ $$aDepartment of Molecular Microbiology and Immunology$$041429 000000430 7001_ $$aNiemann, George$$uOregon Health and Science University$$041354 000000430 7201_ $$aHeffron, Fred$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor$$eMentor 000000430 8564_ $$946cf5e36-4d7b-44a1-9b77-0018e35bf5c6$$s6101357$$uhttps://digitalcollections.ohsu.edu/record/430/files/431_etd.pdf$$ePublic$$2099592bd090c3fd5a51e0743001383e7$$31 000000430 905__ $$a/rest/prod/83/36/h1/91/8336h191v 000000430 909CO $$ooai:digitalcollections.ohsu.edu:430$$pstudent-work 000000430 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument 000000430 980__ $$aTheses and Dissertations