000043400 001__ 43400 000043400 005__ 20240617125020.0 000043400 0247_ $$2doi$$a10.6083/bpxhc43400 000043400 037__ $$aETD 000043400 041__ $$aeng 000043400 245__ $$aProfiling of intrinsically photosensitive retinal ganglion cells using intersectional genetics and single-molecule fluorescence in situ hybridization 000043400 260__ $$bOregon Health and Science University 000043400 269__ $$a2024-06-15 000043400 336__ $$aDissertation 000043400 502__ $$bPh.D. 000043400 502__ $$gNeuroscience 000043400 520__ $$aUnderstanding the molecular and genetic identity of neuron types is prerequisite to tractable control and investigation of the behavioral relevance of discrete neuronal types. In the retina, a portion of retinal ganglion cells (RGCs), express the light-sensitive protein melanopsin, allowing them to detect light independent of rod and cone photoreceptors. These intrinsically photosensitive retinal ganglion cells (ipRGCs), detect light information and transmit the encoded information to brain areas responsible for nonimage forming behaviors, like circadian entrainment, pupillary light reflex, hormone regulation, and sleep. IpRGCs can be divided into types, based upon differences in their morphology, light responses, and brain projections. Understanding the specific functions and contributions of ipRGC types is critical to our understanding of physiology. This dissertation describes the investigation and characterization of a unique population of retinal ganglion cells, which are labeled in a glycine transporter 2 transgenic mouse line (Glyt2 Cre) and restricted to the dorsal retina. The experiments aimed to extend these findings by employing various advanced techniques to label and characterize these cells, including patch-clamp electrophysiology, intersectional genetic labeling, single-cell reverse transcriptase PCR, and fluorescence in situ hybridization (FISH). The initial focus was on validating the expression of Slc6a5 (Glyt2) mRNA in ipRGCs. The evolution of these techniques shifted emphasis toward combining FISH with transgenic reporter lines to label and spatially resolve RGC subtypes across entire retinas. This approach was further applied to identify ipRGC subtypes in non-human primate (NHP) retina, providing broader insights into the molecular identity, spatial distribution, and function of RGC subtypes. 000043400 540__ $$fCC BY-NC 000043400 542__ $$fIn copyright - single owner 000043400 650__ $$aRetinal Ganglion Cells$$025392 000043400 650__ $$aMice, Transgenic$$022253 000043400 650__ $$aIn Situ Hybridization, Fluorescence$$029944 000043400 650__ $$aRetina$$025387 000043400 650__ $$aPrimates$$024593 000043400 650__ $$aChromosomes, Artificial, Bacterial$$032947 000043400 650__ $$aElectrophysiology$$018311 000043400 6531_ $$aipRGC 000043400 6531_ $$amelanopsin 000043400 6531_ $$aOpn4 000043400 691__ $$aSchool of Medicine$$041369 000043400 692__ $$aCasey Eye Institute$$041381 000043400 692__ $$aVollum Institute$$041509 000043400 7001_ $$aGarrett, Tavita$$uOregon Health and Science University$$041354$$10000-0002-2585-4251 000043400 7201_ $$aSivyer, Benjamin$$uOregon Health and Science University$$041354$$10000-0002-4537-5768$$ePI$$7Personal 000043400 7201_ $$aSchnell, Eric$$uOregon Health and Science University$$041354$$10000-0002-5623-5015$$eCommittee chair$$7Personal 000043400 7201_ $$aMorgans, Catherine$$uOregon Health and Science University$$10000-0002-7287-3447$$eCommittee member$$7Personal 000043400 7201_ $$aMishra, Anusha $$uOregon Health and Science University$$041354$$10000-0002-3642-5049$$eCommittee member$$7Personal 000043400 7201_ $$aVon Gersdorff, Henrique$$uOregon Health and Science University$$041354$$10000-0002-4404-3307$$eCommittee member$$7Personal 000043400 7201_ $$aTrussell, Laurence$$uOregon Health and Science University$$041354$$10000-0003-1171-2356$$ePI$$7Personal 000043400 8564_ $$90c63483c-5ce7-40b4-88ac-67c5be812db8$$s22088157$$uhttps://digitalcollections.ohsu.edu/record/43400/files/Garrett.Tavita.2024.pdf 000043400 909CO $$ooai:digitalcollections.ohsu.edu:43400$$pstudent-work 000043400 980__ $$aTheses and Dissertations 000043400 981__ $$aPublished$$b2024-06-17