000043432 001__ 43432
000043432 005__ 20240624125109.0
000043432 037__ $$aETD
000043432 041__ $$aeng
000043432 245__ $$aIdentification of peptidoglycan recognition protein 3 coated microbes in subgingival dental plaque in periodontitis
000043432 260__ $$bOregon Health and Science University
000043432 269__ $$a2023-11-08
000043432 336__ $$aThesis
000043432 502__ $$bM.S.
000043432 502__ $$gPeriodontics
000043432 520__ $$aPGRP3 is a protein that helps the body recognize and respond to bacteria based on the essential structures of the bacteria. They are believed to act by helping to maintain a beneficial microbial community. These proteins can attach to bacteria and coat them. However, it is unknown which bacteria they bind to. Here we separated the PGRP3 coated bacteria from dental plaque samples collected from individuals with severe periodontitis and individuals who did not have a diagnosis of periodontitis (non-periodontitis). We used an antibody-based separation technique to purify PGRP3 coated bacteria to a high level of purity. We then sequenced the DNA of the separated bacteria to identify species. We found that samples obtained from non-periodontitis subjects did not have enough PGRP3 coated bacteria to allow for purification. However, the periodontitis samples had significant numbers of PGRP3 coated bacteria. We also identified the following species as PGRP3 highly coated, i.e., Porphyromonas gingivalis, Corynebacterium durum, Streptococcus mutans, and Neisseria. Sp. Species that are not coated with PGRP3 include the following, Leptotrichia wadei, Selenomonas artemidis, Prevotella salivae, and Leptotrichia shahii. While it appears that PGRP3 coats putative oral pathogens including Porphyromonas gingivalis and Streptococcus mutans, PGRP3 coating is noted in only one of the three Treponema species, suggesting a species-level specificity of PGRP3 binding.
000043432 536__ $$oNIDCR/NIH $$cDE031013
000043432 540__ $$fPublic Domain
000043432 542__ $$fPublic domain
000043432 650__ $$aChronic Periodontitis$$038175
000043432 650__ $$aPeriodontitis$$023830
000043432 650__ $$aDental Plaque$$017526
000043432 650__ $$aImmunity, Innate$$020672
000043432 650__ $$ahost response
000043432 6531_ $$across sectional
000043432 6531_ $$adental microbial biofilm
000043432 6531_ $$apeptidoglycan recognition protein
000043432 6531_ $$apattern recognition protein
000043432 6531_ $$ahost response
000043432 691__ $$aSchool of Dentistry$$041368
000043432 692__ $$aDepartment of Periodontology$$041440
000043432 7001_ $$aYang, Jing I.$$uOregon Health and Science University$$041354
000043432 7001_ $$aMerritt, Justin $$uOregon Health and Science University$$041354
000043432 7001_ $$aKreth, Jens $$uOregon Health and Science University$$041354
000043432 7001_ $$aGanesan, Sukirth $$uThe University of Iowa
000043432 7001_ $$aDabdoub, Shareef M. $$uThe University of Iowa
000043432 7001_ $$aLin, Li-Jung$$uOregon Health and Science University$$041354
000043432 7001_ $$aPrakasam, Sivaraman $$uThe University of Iowa
000043432 7201_ $$aKatancik, Jim $$uOregon Health and Science University$$041354$$eCommittee member$$7Personal
000043432 7201_ $$aMarucha, Phillip T. $$uOregon Health and Science University$$041354$$eCommittee member$$7Personal
000043432 789__ $$eCites
000043432 8564_ $$981b9b9ac-ae40-4c16-8230-4b8d06a1fa7d$$s2886278$$uhttps://digitalcollections.ohsu.edu/record/43432/files/Yang.Jing2024.pdf
000043432 909CO $$ooai:digitalcollections.ohsu.edu:43432$$pstudent-work
000043432 980__ $$aTheses and Dissertations
000043432 981__ $$aPublished$$b2024-06-24