000043628 001__ 43628
000043628 005__ 20240822151226.0
000043628 0247_ $$2doi$$a10.6083/bpxhc43628
000043628 037__ $$aIR
000043628 041__ $$aeng
000043628 245__ $$aCluster of expressed proteins contribute to wildtype PEAR1 function in acute myeloid leukemia cell lines
000043628 260__ $$bOregon Health and Science University
000043628 269__ $$a2024
000043628 336__ $$aAbstract
000043628 520__ $$aThe mutational status of platelet-receptor PEAR1 was recently identified as a major factor for survival of young patients with Acute Myeloid Leukemia. While studies show that wildtype PEAR1 regulates hematopoiesis, little is known on how PEAR1’s mutational status may impact downstream pathway proteins’ activity. We generated triplicate Ba/F3 cell lines using retroviral vector transduction, stably expressing empty vector, wildtype, or mutated PEAR1. Further experiments will help validate the changes in identified pathway proteins across lines using Western blots. Subsequent drug treatments will evaluate the impact of PEAR1’s mutational status on cell survival.
000043628 540__ $$fCC BY
000043628 542__ $$fIn copyright - joint owners
000043628 650__ $$aProteomics$$035059
000043628 6531_ $$aPEAR1
000043628 6531_ $$aacute myeloid leukemia
000043628 6531_ $$areverse-phase protein array
000043628 691__ $$aSchool of Medicine$$041369
000043628 692__ $$aDepartment of Cell, Developmental and Cancer Biology$$041399
000043628 7001_ $$aSax, Luca$$uOregon Health and Science University$$041354
000043628 7001_ $$aTognon, Cristina E.$$uOregon Health and Science University$$041354
000043628 7001_ $$aJoshi, Sunil K.$$uOregon Health and Science University$$041354
000043628 7001_ $$aFernandez, Thomas J.$$uOregon Health and Science University$$041354
000043628 7001_ $$aTyner, Jeffrey W.$$uOregon Health and Science University$$041354
000043628 711__ $$aResearch Week$$uOregon Health and Science University$$d2024
000043628 8564_ $$95e3b8ec8-6b58-4819-82ad-eb438103db77$$s180167$$uhttps://digitalcollections.ohsu.edu/record/43628/files/ResearchWeek.2024.Sax.Luca.pdf
000043628 980__ $$aResearch Week