000043706 001__ 43706 000043706 005__ 20240919130343.0 000043706 0247_ $$2doi$$a10.6083/bpxhc43706 000043706 037__ $$aETD 000043706 041__ $$aeng 000043706 245__ $$aSpatiotemporal gradient of reactive astrogliosis reflects diminished neurovascular coupling after stroke 000043706 260__ $$bOregon Health and Science University 000043706 269__ $$a9/16/2024 000043706 336__ $$aDissertation 000043706 502__ $$bPh.D. 000043706 502__ $$gNeuroscience 000043706 520__ $$aIschemic stroke is the most common type of stroke, accounting for about 87% of all stroke cases. Stroke is a leading cause of long-term disability and the second leading cause of death globally. Understanding the pathophysiology of ischemic stroke can improve early diagnosis and treatment, reduce neuronal damage, and improve recovery. Reactive astrogliosis and microglia are key features of stroke pathophysiology and may contribute to both neuroprotection and neurodysfunction. Here, we map the spatiotemporal progression of reactive astrogliosis through GFAP and vimentin labeling, and reactive microglia through Iba1 labeling. We found that reactive astrogliosis progresses faster and is more widespread than reactive microgliosis and that the astrocyte response is not entirely dependent on microglia. This response appears exacerbated in aging animals. This work also details preliminary findings linking reactive astrogliosis to impaired neurovascular coupling mechanisms after stroke and how this relates to developmental neurovascular coupling mechanisms. 000043706 536__ $$oLacroute Fellowship 000043706 536__ $$oNIA$$cT32AG055378 000043706 536__ $$oNINDS$$cR01NS110690 000043706 540__ $$fCC BY 000043706 542__ $$fIn copyright - single owner 000043706 650__ $$aIschemic Stroke$$013354 000043706 650__ $$aNeurovascular Coupling$$011379 000043706 650__ $$aAstrocytes$$015172 000043706 650__ $$aMicroglia$$030120 000043706 650__ $$aNeurovascular Coupling$$011379 000043706 6531_ $$areactive astrocytes 000043706 6531_ $$areactive microglia 000043706 691__ $$aSchool of Medicine$$041369 000043706 692__ $$aVollum Institute$$041509 000043706 7001_ $$aStackhouse, Teresa$$uOregon Health and Science University$$041354$$10000000229242121 000043706 789__ $$eHas part$$whttps://doi.org/10.3389/fcell.2021.702832$$2DOI 000043706 792__ $$aStackhouse, T. L., & Mishra, A. (2021). Neurovascular Coupling in Development and Disease: Focus on Astrocytes. Frontiers in cell and developmental biology, 9, 702832. https://doi.org/10.3389/fcell.2021.702832 000043706 8564_ $$971b69515-0202-4c3f-8eee-ede41213ffe3$$s8098028$$uhttps://digitalcollections.ohsu.edu/record/43706/files/Stackhouse.Teresa.2024.pdf 000043706 909CO $$ooai:digitalcollections.ohsu.edu:43706$$pstudent-work 000043706 980__ $$aTheses and Dissertations 000043706 981__ $$aPublished$$b2024-09-18