TY  - GEN
AB  - Tissue regeneration requires carefully balanced cell proliferation, and our previous work shows that fusion between bone marrow–derived cells (BMDCs) and intestinal epithelial cells occurs after injury as a potential repair mechanism. This thesis investigates the environmental cues and cell types that drive intestinal cell fusion and examines the properties of resulting fusion hybrids. Using transplantation and parabiosis models, I identify local inflammation and epithelial hyperproliferation as key mediators of fusion. Donor immune cells—primarily macrophages—cluster around injured crypts before fusion occurs. Transcriptome analysis reveals that fusion hybrids retain both epithelial and macrophage signatures. These findings demonstrate that cell fusion generates unique hybrid cells with potential roles in regeneration and tumorigenesis and suggest a mechanistic link between intestinal inflammation and cancer.
AD  - Oregon Health and Science University
AU  - Powell, Anne
DA  - 2010
DO  - 10.6083/M44M92JP
DO  - DOI
ID  - 439
KW  - Mice
KW  - Stem Cells
KW  - Blood Cells
KW  - Cell Fusion
KW  - Intestinal Mucosa
KW  - Bone Marrow
KW  - Cell Proliferation
L1  - https://digitalcollections.ohsu.edu/record/439/files/440_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/439/files/440_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/439/files/440_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/439/files/440_etd.pdf
N2  - Tissue regeneration requires carefully balanced cell proliferation, and our previous work shows that fusion between bone marrow–derived cells (BMDCs) and intestinal epithelial cells occurs after injury as a potential repair mechanism. This thesis investigates the environmental cues and cell types that drive intestinal cell fusion and examines the properties of resulting fusion hybrids. Using transplantation and parabiosis models, I identify local inflammation and epithelial hyperproliferation as key mediators of fusion. Donor immune cells—primarily macrophages—cluster around injured crypts before fusion occurs. Transcriptome analysis reveals that fusion hybrids retain both epithelial and macrophage signatures. These findings demonstrate that cell fusion generates unique hybrid cells with potential roles in regeneration and tumorigenesis and suggest a mechanistic link between intestinal inflammation and cancer.
PB  - Oregon Health and Science University
PY  - 2010
T1  - Cellular fusion in the mouse intestine
TI  - Cellular fusion in the mouse intestine
UR  - https://digitalcollections.ohsu.edu/record/439/files/440_etd.pdf
Y1  - 2010
ER  -