000000439 001__ 439
000000439 005__ 20260203145359.0
000000439 0247_ $$2DOI$$a10.6083/M44M92JP
000000439 037__ $$aETD
000000439 245__ $$aCellular fusion in the mouse intestine
000000439 260__ $$bOregon Health and Science University
000000439 269__ $$a2010
000000439 336__ $$aDissertation
000000439 502__ $$bPh.D.
000000439 520__ $$aTissue regeneration requires carefully balanced cell proliferation, and our previous work shows that fusion between bone marrow–derived cells (BMDCs) and intestinal epithelial cells occurs after injury as a potential repair mechanism. This thesis investigates the environmental cues and cell types that drive intestinal cell fusion and examines the properties of resulting fusion hybrids. Using transplantation and parabiosis models, I identify local inflammation and epithelial hyperproliferation as key mediators of fusion. Donor immune cells—primarily macrophages—cluster around injured crypts before fusion occurs. Transcriptome analysis reveals that fusion hybrids retain both epithelial and macrophage signatures. These findings demonstrate that cell fusion generates unique hybrid cells with potential roles in regeneration and tumorigenesis and suggest a mechanistic link between intestinal inflammation and cancer.
000000439 540__ $$fCC BY
000000439 542__ $$fIn copyright - single owner
000000439 650__ $$aMice$$036842
000000439 650__ $$aStem Cells$$026391
000000439 650__ $$aBlood Cells$$015648
000000439 650__ $$aCell Fusion$$016283
000000439 650__ $$aIntestinal Mucosa$$020954
000000439 650__ $$aBone Marrow$$015725
000000439 650__ $$aCell Proliferation$$036166
000000439 691__ $$aSchool of Medicine$$041369
000000439 692__ $$aDepartment of Cell, Developmental and Cancer Biology$$041399
000000439 7001_ $$aPowell, Anne$$uOregon Health and Science University$$041354
000000439 8564_ $$9808a320a-5aa1-4b49-9f6e-0e8b3d0ccd84$$s13136160$$uhttps://digitalcollections.ohsu.edu/record/439/files/440_etd.pdf$$ePublic$$213cc29b646077b552a0071eab3a03f11$$31
000000439 905__ $$a/rest/prod/nz/80/5z/71/nz805z711
000000439 909CO $$ooai:digitalcollections.ohsu.edu:439$$pstudent-work
000000439 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000439 980__ $$aTheses and Dissertations