TY  - GEN
AB  - The goal of my PhD has been to develop and adapt optical chemical biology approaches to study signal transduction in cells. I developed genetically encoded FRET sensors to monitor with high temporal resolution PARP1-dependent ADP-ribosylation in live cells. I explored CB1 location-dependent functionality by adapting genetic code expansion to generate novel tagging strategies in live cells. Finally, I characterized chemically engineered lipid probes designed to follow lipid transport, metabolism and protein-interactions. 
AD  - Oregon Health and Science University
AU  - Thomas, Alix
DA  - 2024-10-31
DO  - 10.6083/bpxhc43998
DO  - doi
ID  - 43998
KW  - Signal Transduction
KW  - Fluorescence Resonance Energy Transfer
KW  - ADP-Ribosylation
KW  - Lipids
KW  - Receptor, Cannabinoid, CB1
KW  - chemical biology
KW  - FRET 
KW  - ADPr
KW  -  CB1
L1  - https://digitalcollections.ohsu.edu/record/43998/files/Thomas.Alix.2024.pdf
L2  - https://digitalcollections.ohsu.edu/record/43998/files/Thomas.Alix.2024.pdf
L4  - https://digitalcollections.ohsu.edu/record/43998/files/Thomas.Alix.2024.pdf
LA  - eng
LK  - https://digitalcollections.ohsu.edu/record/43998/files/Thomas.Alix.2024.pdf
N2  - The goal of my PhD has been to develop and adapt optical chemical biology approaches to study signal transduction in cells. I developed genetically encoded FRET sensors to monitor with high temporal resolution PARP1-dependent ADP-ribosylation in live cells. I explored CB1 location-dependent functionality by adapting genetic code expansion to generate novel tagging strategies in live cells. Finally, I characterized chemically engineered lipid probes designed to follow lipid transport, metabolism and protein-interactions. 
PB  - Oregon Health and Science University
PY  - 2024-10-31
T1  - Development of chemical biology tools to follow signal transduction in cells
TI  - Development of chemical biology tools to follow signal transduction in cells
UR  - https://digitalcollections.ohsu.edu/record/43998/files/Thomas.Alix.2024.pdf
Y1  - 2024-10-31
ER  -