TY - THES AB - Novel anti-CB1 monoclonal antibodies, including an N-terminal binding antibody, were characterized and validated. These antibodies were then used to investigate the role of the CB1 N-terminus in subcellular localization. Finally, the ability of one antibody was assessed for immunoaffinity purification. In this thesis, I detail the characterization and validation of several novel monoclonal anti-CB1 antibodies as well as several applications of these antibodies. AD - Oregon Health and Science University AU - Matheson, Triona DA - 2024-12-02 DO - 10.6083/bpxhc44092 DO - doi ID - 44092 KW - Receptor, Cannabinoid, CB1 KW - Antibodies, Monoclonal KW - Receptors, G-Protein-Coupled KW - GPCR L1 - https://digitalcollections.ohsu.edu/record/44092/files/Matheson.Triona.2024.pdf L2 - https://digitalcollections.ohsu.edu/record/44092/files/Matheson.Triona.2024.pdf L4 - https://digitalcollections.ohsu.edu/record/44092/files/Matheson.Triona.2024.pdf LA - eng LK - https://digitalcollections.ohsu.edu/record/44092/files/Matheson.Triona.2024.pdf N2 - Novel anti-CB1 monoclonal antibodies, including an N-terminal binding antibody, were characterized and validated. These antibodies were then used to investigate the role of the CB1 N-terminus in subcellular localization. Finally, the ability of one antibody was assessed for immunoaffinity purification. In this thesis, I detail the characterization and validation of several novel monoclonal anti-CB1 antibodies as well as several applications of these antibodies. PB - Oregon Health and Science University PY - 2024-12-02 T1 - Characterization and validation of novel anti-CB1 monoclonal antibodies and their application to GPCR studies TI - Characterization and validation of novel anti-CB1 monoclonal antibodies and their application to GPCR studies UR - https://digitalcollections.ohsu.edu/record/44092/files/Matheson.Triona.2024.pdf Y1 - 2024-12-02 ER -