000044092 001__ 44092 000044092 005__ 20241220100042.0 000044092 0247_ $$2doi$$a10.6083/bpxhc44092 000044092 037__ $$aETD 000044092 041__ $$aeng 000044092 245__ $$aCharacterization and validation of novel anti-CB1 monoclonal antibodies and their application to GPCR studies 000044092 260__ $$bOregon Health and Science University 000044092 269__ $$a2024-12-02 000044092 336__ $$aThesis 000044092 502__ $$gBiochemistry & Molecular Biology (sunsetting) 000044092 502__ $$bM.S. 000044092 520__ $$aNovel anti-CB1 monoclonal antibodies, including an N-terminal binding antibody, were characterized and validated. These antibodies were then used to investigate the role of the CB1 N-terminus in subcellular localization. Finally, the ability of one antibody was assessed for immunoaffinity purification. In this thesis, I detail the characterization and validation of several novel monoclonal anti-CB1 antibodies as well as several applications of these antibodies. 000044092 536__ $$oNIDA$$c5T32DA007262-29 000044092 540__ $$fCC BY 000044092 542__ $$fIn copyright - single owner 000044092 650__ $$aReceptor, Cannabinoid, CB1$$035348 000044092 650__ $$aAntibodies, Monoclonal$$014854 000044092 650__ $$aReceptors, G-Protein-Coupled$$035314 000044092 6531_ $$aGPCR 000044092 691__ $$aSchool of Medicine$$041369 000044092 692__ $$aDepartment of Biochemistry and Molecular Biology$$041396 000044092 7001_ $$aMatheson, Triona$$uOregon Health and Science University$$041354$$10000-0002-7764-909X 000044092 8564_ $$uhttps://digitalcollections.ohsu.edu/record/44092/files/Matheson.Triona.2024.pdf$$9111e7dc0-3ee9-4228-8190-2ba33abc5191$$s4817239 000044092 909CO $$ooai:digitalcollections.ohsu.edu:44092$$pstudent-work 000044092 980__ $$aTheses and Dissertations 000044092 981__ $$aPublished$$b2024-12-06