This thesis investigates the development and application of spectrally distinct fluorescent probes for fluorescence-guided surgery (FGS) to improve intraoperative visualization of tumors and critical nerve structures during head and neck cancer surgeries. A near-infrared (700 nm) nerve-specific fluorophore was paired with an 800 nm tumor-targeting probe to enable simultaneous, real-time delineation of healthy and malignant tissues. The work evaluates probe performance, specificity, and pharmacokinetics in preclinical models, demonstrating the potential of dual-wavelength imaging to enhance surgical precision and functional outcomes.
