000000450 001__ 450
000000450 005__ 20260410124447.0
000000450 0247_ $$2DOI$$a10.6083/M4QC01FC
000000450 037__ $$aETD
000000450 245__ $$aCloning and characterization of Xenopus laevis proprotein convertases and identification of endogenous convertases of bone morphogenetic protein 4
000000450 260__ $$bOregon Health and Science University
000000450 269__ $$a2009
000000450 336__ $$aDissertation
000000450 502__ $$bPh.D.
000000450 520__ $$aBMP4, a highly conserved TGFβ family ligand, requires proteolytic cleavage by proprotein convertases (PCs) for activation. ProBMP4 is processed first at the S1 site and then at the upstream S2 site, which regulates BMP4 activity in a tissue‑specific manner. Using Xenopus, we identified the PCs responsible for these cleavages: furin and PC6 cleave both S1 and S2, while a third enzyme, likely PC7, selectively cleaves S1 in embryos. These findings suggest that constitutive S1 cleavage by broadly expressed PC7, combined with S1+S2 cleavage by tissue‑restricted furin or PC6, enables tissue‑specific regulation of BMP4 signaling.
000000450 540__ $$fCC BY
000000450 542__ $$fIn copyright - single owner
000000450 650__ $$aXenopus Proteins$$033748
000000450 650__ $$aXenopus laevis$$028036
000000450 650__ $$aBone Morphogenetic Protein 4$$038290
000000450 650__ $$aProprotein Convertases$$035308
000000450 650__ $$aPC6 extract
000000450 650__ $$aTransforming Growth Factors$$028296
000000450 691__ $$aSchool of Medicine$$041369
000000450 692__ $$aDepartment of Molecular and Medical Genetics$$041428
000000450 7001_ $$aNelsen, Sylvia$$uOregon Health and Science University$$041354
000000450 7201_ $$aChristian, Jan$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000450 8564_ $$952e5160a-e607-419c-b688-e39827162866$$s2244603$$uhttps://digitalcollections.ohsu.edu/record/450/files/451_etd.pdf$$ePublic$$227b6b49dfbc963137a8ccb81ca1b3222$$31
000000450 901__ $$a<p>These documents are archival records. They are retained for historical reference only. </p><p><b>Need an accessible version? Use the ‘Get Accessible Copy’ link above.</b></p>
000000450 905__ $$a/rest/prod/h7/02/q6/37/h702q637s
000000450 909CO $$ooai:digitalcollections.ohsu.edu:450$$pstudent-work
000000450 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000450 980__ $$aTheses and Dissertations