Malaria infection during pregnancy remains a major public health concern in the WHO African Region, with an estimated 13 million pregnancies affected by malaria across 33 endemic countries in 2024. On Bioko Island, Equatorial Guinea, sustained malaria control efforts have substantially reduced transmission, while gaps in the uptake of recommended prevention behaviors among pregnant women persist. This dissertation examines malaria prevention during pregnancy, including intermittent preventive treatment, antenatal care attendance, and mosquito net use, and evaluates psychosocial and knowledge-related factors associated with the uptake of prevention behaviors and their relationship with maternal clinical diagnoses. Using survey data collected from pregnant and postpartum women and routine antenatal care records, this research addresses gaps in understanding malaria prevention during pregnancy and opportunities to strengthen prevention efforts on Bioko Island.