000000461 001__ 461
000000461 005__ 20260203152912.0
000000461 0247_ $$2DOI$$a10.6083/M4959FJ9
000000461 037__ $$aETD
000000461 245__ $$aCollagen and collagen-associated proteins studies on the type XV trimerization domain, prolyl 3-hydrolase and the prolyl 3-hydroxylase cartilage associated protein cyclophilin B complex
000000461 260__ $$bOregon Health and Science University
000000461 269__ $$a2010
000000461 336__ $$aDissertation
000000461 502__ $$bPh.D.
000000461 502__ $$gBiochemistry & Molecular Biology (sunsetting)
000000461 520__ $$aCollagen and its associated proteins are essential for proper tissue structure, and defects in either can lead to numerous diseases. While collagen triple‑helical domains are well studied, much less is known about their non‑collagenous regions. This work reports the crystal structure and biochemical properties of the non‑collagenous trimerization domain of human type XV collagen, revealing a highly stable trimer supported by hydrophobic interactions within and between monomers, capable of forming at picomolar concentrations. We also show that the endoplasmic reticulum P3H1●CRTAP●CypB complex functions as a prolyl 3‑hydroxylase, molecular chaperone, and protein disulfide isomerase, binding both unfolded and triple‑helical collagen. Despite efforts to improve yields, the complex remains unstable, limiting deeper structural characterization. These findings expand understanding of collagen assembly and the multifunctional machinery required for its biosynthesis.
000000461 540__ $$fCC BY
000000461 542__ $$fIn copyright - single owner
000000461 650__ $$aCollagen$$016890
000000461 650__ $$aExtracellular Matrix Proteins$$029067
000000461 650__ $$aProcollagen-Proline Dioxygenase$$024659
000000461 650__ $$aProtein Multimerization$$038334
000000461 650__ $$aCyclophilins$$032917
000000461 6531_ $$acyclophilin b
000000461 6531_ $$atrimerization domain
000000461 6531_ $$atype xv collagen
000000461 6531_ $$acartilage associated protein
000000461 6531_ $$aprolyl 3-hydroxylase
000000461 691__ $$aSchool of Medicine$$041369
000000461 692__ $$aDepartment of Biochemistry and Molecular Biology$$041396
000000461 7001_ $$aWirz, Jacqueline$$uOregon Health and Science University$$041354
000000461 8564_ $$9e073e42f-b453-4dda-a216-d6241f5efaa3$$s1366511$$uhttps://digitalcollections.ohsu.edu/record/461/files/462_etd.pdf$$ePublic$$2820d0f975021fe288af25d9a16b55145$$31
000000461 905__ $$a/rest/prod/41/68/7h/45/41687h45k
000000461 909CO $$ooai:digitalcollections.ohsu.edu:461$$pstudent-work
000000461 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000461 980__ $$aTheses and Dissertations