TY  - GEN
AB  - DNA–protein crosslinks (DPCs) form after exposure to agents such as formaldehyde, yet the pathways that repair or tolerate these lesions are not fully defined. A genome‑wide yeast deletion screen revealed that chronic low‑dose formaldehyde toxicity is primarily mitigated by homologous recombination, whereas acute high‑dose exposure depends on nucleotide excision repair (NER). Findings suggest that acute repair proceeds through NER‑dependent single‑strand break intermediates without detectable double‑strand breaks. Additional analyses show that translesion synthesis acts as a backup pathway and that Mre11 and Rad1 contribute independently to DPC tolerance. These results highlight distinct pathway requirements for chronic versus acute DPC exposure.
AD  - Oregon Health and Science University
AU  - de Graaf, Bendert
DA  - 2009
DO  - 10.6083/M41N7Z38
DO  - DOI
ED  - McCullough, Amanda
ED  - Mentor
ID  - 463
KW  - Cell Survival
KW  - DNA Repair
KW  - Saccharomyces cerevisiae
KW  - Formaldehyde
KW  - DNA
KW  - Formaldehyde poisoning
L1  - https://digitalcollections.ohsu.edu/record/463/files/464_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/463/files/464_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/463/files/464_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/463/files/464_etd.pdf
N2  - DNA–protein crosslinks (DPCs) form after exposure to agents such as formaldehyde, yet the pathways that repair or tolerate these lesions are not fully defined. A genome‑wide yeast deletion screen revealed that chronic low‑dose formaldehyde toxicity is primarily mitigated by homologous recombination, whereas acute high‑dose exposure depends on nucleotide excision repair (NER). Findings suggest that acute repair proceeds through NER‑dependent single‑strand break intermediates without detectable double‑strand breaks. Additional analyses show that translesion synthesis acts as a backup pathway and that Mre11 and Rad1 contribute independently to DPC tolerance. These results highlight distinct pathway requirements for chronic versus acute DPC exposure.
PB  - Oregon Health and Science University
PY  - 2009
T1  - Cellular response pathways to formaldehyde
TI  - Cellular response pathways to formaldehyde
UR  - https://digitalcollections.ohsu.edu/record/463/files/464_etd.pdf
Y1  - 2009
ER  -