000000463 001__ 463
000000463 005__ 20260227103134.0
000000463 0247_ $$2DOI$$a10.6083/M41N7Z38
000000463 037__ $$aETD
000000463 245__ $$aCellular response pathways to formaldehyde
000000463 260__ $$bOregon Health and Science University
000000463 269__ $$a2009
000000463 336__ $$aDissertation
000000463 502__ $$bPh.D.
000000463 520__ $$aDNA–protein crosslinks (DPCs) form after exposure to agents such as formaldehyde, yet the pathways that repair or tolerate these lesions are not fully defined. A genome‑wide yeast deletion screen revealed that chronic low‑dose formaldehyde toxicity is primarily mitigated by homologous recombination, whereas acute high‑dose exposure depends on nucleotide excision repair (NER). Findings suggest that acute repair proceeds through NER‑dependent single‑strand break intermediates without detectable double‑strand breaks. Additional analyses show that translesion synthesis acts as a backup pathway and that Mre11 and Rad1 contribute independently to DPC tolerance. These results highlight distinct pathway requirements for chronic versus acute DPC exposure.
000000463 540__ $$fCC BY
000000463 542__ $$fIn copyright - single owner
000000463 650__ $$aCell Survival$$016294
000000463 650__ $$aDNA Repair$$017990
000000463 650__ $$aSaccharomyces cerevisiae$$025647
000000463 650__ $$aFormaldehyde$$019221
000000463 650__ $$aDNA$$017977
000000463 650__ $$aFormaldehyde poisoning
000000463 691__ $$aSchool of Medicine$$041369
000000463 692__ $$aDepartment of Molecular and Medical Genetics$$041428
000000463 7001_ $$ade Graaf, Bendert$$uOregon Health and Science University$$041354
000000463 7201_ $$aMcCullough, Amanda$$uOregon Health and Science University$$041354$$7Personal$$eMentor
000000463 8564_ $$990895077-cf23-4384-a496-372cd4a53352$$s2741473$$uhttps://digitalcollections.ohsu.edu/record/463/files/464_etd.pdf$$ePublic$$2e2b16cc33b74564a2da9bbdef2daad51$$31
000000463 905__ $$a/rest/prod/79/40/7x/16/79407x16z
000000463 909CO $$ooai:digitalcollections.ohsu.edu:463$$pstudent-work
000000463 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000463 980__ $$aTheses and Dissertations