TY  - GEN
AB  - Glutamate, the primary excitatory neurotransmitter in the brain, must be rapidly cleared from the extracellular space to prevent excitotoxicity and maintain precise synaptic signaling. This work examined how high‑affinity, Na⁺‑dependent glutamate transporters regulate extracellular glutamate levels in hippocampal tissue. Experiments in rodent brain slices showed that ambient glutamate is maintained at ~25 nM, below the activation threshold of most receptors, and that transporter activity is essential for sustaining these low levels. Additional analyses revealed that ambient glutamate is uniformly low throughout the neuropil, indicating that dense, widespread transporter expression prevents tonic activation of both synaptic and extrasynaptic receptors.
AD  - Oregon Health and Science University
AU  - Herman, Melissa
DA  - 2009
DO  - 10.6083/M4GH9FX7
DO  - DOI
ED  - Jahr, Craig
ED  - Advisor
ID  - 506
KW  - Glutamic Acid
KW  - Glutamate Plasma Membrane Transport Proteins
KW  - Synaptic Transmission
KW  - Hippocampus
KW  - Receptors, Glutamate
KW  - Central Nervous System
KW  - Extracellular Space
KW  - Glutamates
KW  - Receptors, N-Methyl-D-Aspartate
L1  - https://digitalcollections.ohsu.edu/record/506/files/507_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/506/files/507_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/506/files/507_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/506/files/507_etd.pdf
N2  - Glutamate, the primary excitatory neurotransmitter in the brain, must be rapidly cleared from the extracellular space to prevent excitotoxicity and maintain precise synaptic signaling. This work examined how high‑affinity, Na⁺‑dependent glutamate transporters regulate extracellular glutamate levels in hippocampal tissue. Experiments in rodent brain slices showed that ambient glutamate is maintained at ~25 nM, below the activation threshold of most receptors, and that transporter activity is essential for sustaining these low levels. Additional analyses revealed that ambient glutamate is uniformly low throughout the neuropil, indicating that dense, widespread transporter expression prevents tonic activation of both synaptic and extrasynaptic receptors.
PB  - Oregon Health and Science University
PY  - 2009
T1  - Control of extracellular glutamate by transporters in the CNS
TI  - Control of extracellular glutamate by transporters in the CNS
UR  - https://digitalcollections.ohsu.edu/record/506/files/507_etd.pdf
Y1  - 2009
ER  -