000000506 001__ 506
000000506 005__ 20260227110746.0
000000506 0247_ $$2DOI$$a10.6083/M4GH9FX7
000000506 037__ $$aETD
000000506 245__ $$aControl of extracellular glutamate by transporters in the CNS
000000506 260__ $$bOregon Health and Science University
000000506 269__ $$a2009
000000506 336__ $$aDissertation
000000506 502__ $$bPh.D.
000000506 502__ $$gNeuroscience
000000506 520__ $$aGlutamate, the primary excitatory neurotransmitter in the brain, must be rapidly cleared from the extracellular space to prevent excitotoxicity and maintain precise synaptic signaling. This work examined how high‑affinity, Na⁺‑dependent glutamate transporters regulate extracellular glutamate levels in hippocampal tissue. Experiments in rodent brain slices showed that ambient glutamate is maintained at ~25 nM, below the activation threshold of most receptors, and that transporter activity is essential for sustaining these low levels. Additional analyses revealed that ambient glutamate is uniformly low throughout the neuropil, indicating that dense, widespread transporter expression prevents tonic activation of both synaptic and extrasynaptic receptors.
000000506 540__ $$fCC BY
000000506 542__ $$fIn copyright - single owner
000000506 650__ $$aGlutamic Acid$$031020
000000506 650__ $$aGlutamate Plasma Membrane Transport Proteins$$036392
000000506 650__ $$aSynaptic Transmission$$022820
000000506 650__ $$aHippocampus$$020217
000000506 650__ $$aReceptors, Glutamate$$029996
000000506 650__ $$aCentral Nervous System$$016312
000000506 650__ $$aExtracellular Space$$018797
000000506 650__ $$aGlutamates$$019605
000000506 650__ $$aReceptors, N-Methyl-D-Aspartate$$028954
000000506 691__ $$aSchool of Medicine$$041369
000000506 692__ $$aVollum Institute$$041509
000000506 7001_ $$aHerman, Melissa$$uOregon Health and Science University$$041354
000000506 7201_ $$aJahr, Craig$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000506 8564_ $$9f7b1a463-c06e-4c0c-ab96-2b64bff7618e$$s735765$$uhttps://digitalcollections.ohsu.edu/record/506/files/507_etd.pdf$$ePublic$$2b7ac76a8ba78ad2a92f986eecc3f7e2e$$31
000000506 905__ $$a/rest/prod/sx/61/dm/29/sx61dm295
000000506 909CO $$ooai:digitalcollections.ohsu.edu:506$$pstudent-work
000000506 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000506 980__ $$aTheses and Dissertations