TY  - GEN
AB  - Francisella tularensis is a highly infectious bacterium with poorly defined mechanisms of pathogenesis. This thesis develops a transposon mutagenesis system for F. tularensis subsp. novicida, enabling identification of several novel virulence factors. Immunization with mutant derivatives of four factors protected mice from high‑dose wild‑type challenge, highlighting vaccine potential. One disrupted gene encodes tandem repeat motifs; a single repeat forms a stable, oligomeric domain that binds host E‑cadherin–associated factors and resembles adhesion domains in Staphylococcus aureus. These findings advance understanding of Francisella virulence, entry, and vaccine development.
AD  - Oregon Health and Science University
AU  - Tempel, Rebecca
DA  - 2008
DO  - 10.6083/M4ST7MSN
DO  - DOI
ED  - Heffron, Fred
ED  - Advisor
ED  - Mentor
ID  - 519
KW  - Francisella
KW  - Virulence Factors
KW  - Tularemia
KW  - Mice
KW  - Immunization
KW  - Vaccines
KW  - Vaccine Development
KW  - Mutagenesis
L1  - https://digitalcollections.ohsu.edu/record/519/files/520_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/519/files/520_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/519/files/520_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/519/files/520_etd.pdf
N2  - Francisella tularensis is a highly infectious bacterium with poorly defined mechanisms of pathogenesis. This thesis develops a transposon mutagenesis system for F. tularensis subsp. novicida, enabling identification of several novel virulence factors. Immunization with mutant derivatives of four factors protected mice from high‑dose wild‑type challenge, highlighting vaccine potential. One disrupted gene encodes tandem repeat motifs; a single repeat forms a stable, oligomeric domain that binds host E‑cadherin–associated factors and resembles adhesion domains in Staphylococcus aureus. These findings advance understanding of Francisella virulence, entry, and vaccine development.
PB  - Oregon Health and Science University
PY  - 2008
T1  - Discovery and analysis of novel Francisella virulence factors
TI  - Discovery and analysis of novel Francisella virulence factors
UR  - https://digitalcollections.ohsu.edu/record/519/files/520_etd.pdf
Y1  - 2008
ER  -