000000519 001__ 519
000000519 005__ 20260410124448.0
000000519 0247_ $$2DOI$$a10.6083/M4ST7MSN
000000519 037__ $$aETD
000000519 245__ $$aDiscovery and analysis of novel Francisella virulence factors
000000519 260__ $$bOregon Health and Science University
000000519 269__ $$a2008
000000519 336__ $$aDissertation
000000519 502__ $$bPh.D.
000000519 520__ $$aFrancisella tularensis is a highly infectious bacterium with poorly defined mechanisms of pathogenesis. This thesis develops a transposon mutagenesis system for F. tularensis subsp. novicida, enabling identification of several novel virulence factors. Immunization with mutant derivatives of four factors protected mice from high‑dose wild‑type challenge, highlighting vaccine potential. One disrupted gene encodes tandem repeat motifs; a single repeat forms a stable, oligomeric domain that binds host E‑cadherin–associated factors and resembles adhesion domains in Staphylococcus aureus. These findings advance understanding of Francisella virulence, entry, and vaccine development.
000000519 540__ $$fCC BY
000000519 542__ $$fIn copyright - single owner
000000519 650__ $$aFrancisella$$019263
000000519 650__ $$aVirulence Factors$$034836
000000519 650__ $$aTularemia$$027499
000000519 650__ $$aMice$$036842
000000519 650__ $$aImmunization$$020673
000000519 650__ $$aVaccines$$027692
000000519 650__ $$aVaccine Development$$013568
000000519 650__ $$aMutagenesis$$029042
000000519 691__ $$aSchool of Medicine$$041369
000000519 692__ $$aDepartment of Molecular Microbiology and Immunology$$041429
000000519 7001_ $$aTempel, Rebecca$$uOregon Health and Science University$$041354
000000519 7201_ $$aHeffron, Fred$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor$$eMentor
000000519 8564_ $$95ae911ee-2b06-4290-8793-ee595f0a6dd8$$s2245435$$uhttps://digitalcollections.ohsu.edu/record/519/files/520_etd.pdf$$ePublic$$2a8076867703311b075094311729a0968$$31
000000519 901__ $$a<p>These documents are archival records. They are retained for historical reference only. </p><p><b>Need an accessible version? Use the ‘Get Accessible Copy’ link above.</b></p>
000000519 905__ $$a/rest/prod/nc/58/0m/67/nc580m674
000000519 909CO $$ooai:digitalcollections.ohsu.edu:519$$pstudent-work
000000519 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000519 980__ $$aTheses and Dissertations