000000527 001__ 527
000000527 005__ 20260226144559.0
000000527 0247_ $$2DOI$$a10.6083/M4RV0KP8
000000527 037__ $$aETD
000000527 245__ $$aA study of the immunological synapses and kinapses formed by CD4 T cell subsets
000000527 260__ $$bOregon Health and Science University
000000527 269__ $$a2009
000000527 336__ $$aDissertation
000000527 502__ $$bPh.D.
000000527 520__ $$aThe immunological synapse (IS) is a structured interface between T cells and antigen‑presenting cells, but its organization varies with T cell differentiation state. This work tests the hypothesis that CD4 T cell subsets with distinct effector functions form structurally different IS. Using transfected fibroblasts, supported planar bilayers, and dendritic cells, I show that Th1 cells form classic centralized IS, while Th2 cells form multifocal synapses with dispersed TCR‑pMHC clusters and altered signaling organization. Induced regulatory T cells (iTregs) form either stable IS or motile kinapses depending on CD80 levels, and they downregulate CD80 on dendritic cells in an antigen‑specific manner. These findings demonstrate that IS architecture is subset‑specific and closely linked to effector function and regulatory activity.
000000527 540__ $$fCC BY
000000527 542__ $$fIn copyright - single owner
000000527 650__ $$aTh1 Cells$$030784
000000527 650__ $$aTh2 Cells$$030785
000000527 650__ $$aT-Lymphocytes$$026731
000000527 650__ $$aImmunological Synapses$$038122
000000527 650__ $$aLipid Bilayers$$021535
000000527 6531_ $$aregulatory
000000527 691__ $$aSchool of Medicine$$041369
000000527 692__ $$aDepartment of Molecular Microbiology and Immunology$$041429
000000527 7001_ $$aThauland, Timothy$$uOregon Health and Science University$$041354
000000527 7201_ $$aParker, David$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor$$eMentor
000000527 8564_ $$9a407bf3d-36d9-4ae4-ab4c-9dba25ef13a0$$s3143836$$uhttps://digitalcollections.ohsu.edu/record/527/files/528_etd.pdf$$ePublic$$2c083886e4708a292b1c09d989d2591b7$$31
000000527 905__ $$a/rest/prod/wm/11/7n/97/wm117n97m
000000527 909CO $$ooai:digitalcollections.ohsu.edu:527$$pstudent-work
000000527 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000527 980__ $$aTheses and Dissertations