000000536 001__ 536
000000536 005__ 20260304123133.0
000000536 0247_ $$2DOI$$a10.6083/M4M61H76
000000536 037__ $$aETD
000000536 245__ $$aThe role of Mad4, Mnt and c-Myc proteins in OX40 stimulated T cells
000000536 260__ $$bOregon Health and Science University
000000536 269__ $$a2009
000000536 336__ $$aThesis
000000536 502__ $$bPh.D.
000000536 520__ $$aThis study investigated the molecular mechanisms through which OX40 stimulation enhances T‑cell function. Gene expression profiling of OX40‑activated antigen‑specific T cells suggested involvement of the transcription factor c‑Myc and its antagonists Mad4 and Mnt. Using an adoptive transfer model of OVA‑specific DO11.10 T cells in BALB/c mice, we found that OX40 engagement increased T‑cell proliferation and survival. Western blot analyses showed that Mad4, Mnt, and c‑Myc were upregulated early after OX40 stimulation, with OX40 signaling stabilizing Mad4 and Mnt proteins by preventing their degradation. siRNA knockdown of Mad4 and Mnt reduced survival of OX40‑activated T cells, indicating these proteins help counterbalance c‑Myc–driven activation and support memory T‑cell development.
000000536 540__ $$fCC BY
000000536 542__ $$fIn copyright - single owner
000000536 650__ $$aT-Lymphocytes$$026731
000000536 650__ $$aOX40 Ligand$$037329
000000536 650__ $$aRepressor Proteins$$025328
000000536 650__ $$aMemory T Cells$$013784
000000536 650__ $$aGene Expression Profiling$$032769
000000536 650__ $$aGene Expression Profiling$$032769
000000536 650__ $$aProto-Oncogene Proteins c-myc$$029021
000000536 691__ $$aSchool of Medicine$$041369
000000536 692__ $$aDepartment of Cell, Developmental and Cancer Biology$$041399
000000536 7001_ $$aVasilevsky, Nicole$$uOregon Health and Science University$$041354
000000536 7201_ $$aWeinberg, Andy$$uOregon Health and Science University$$041354$$7Personal$$eMentor
000000536 8564_ $$9f701566d-8e0d-42e3-876e-4b5d0b0d093b$$s4767014$$uhttps://digitalcollections.ohsu.edu/record/536/files/537_etd.pdf$$ePublic$$2b071bc0140cb15c8216af241b0f8be42$$31
000000536 905__ $$a/rest/prod/g7/32/d9/03/g732d9039
000000536 909CO $$ooai:digitalcollections.ohsu.edu:536$$pstudent-work
000000536 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000536 980__ $$aTheses and Dissertations