000000556 001__ 556
000000556 005__ 20260203141206.0
000000556 0247_ $$2DOI$$a10.6083/M41834GZ
000000556 037__ $$aETD
000000556 245__ $$aATP7B expression and trafficking in the intestine and ovary: insights into specialized function
000000556 260__ $$bOregon Health and Science University
000000556 269__ $$a2010
000000556 336__ $$aThesis
000000556 502__ $$bM.S.
000000556 520__ $$aCopper is essential for cellular function, but excess copper is toxic, requiring tight regulation through copper‑binding proteins and the ATP7A and ATP7B transporters. While ATP7B’s role in hepatic copper export is well established, its functions in other tissues are less understood. This work examines ATP7B expression and trafficking in the human large intestine and ovary. In colonic epithelial cells, ATP7B localizes apically and undergoes copper‑dependent trafficking, supporting a model in which excess copper is packaged into vesicles and excreted into the intestinal lumen. Additionally, using cisplatin‑resistant A2780‑CP20 cells, we show that ATP7B does not traffic in response to copper or cisplatin, suggesting that resistance arises from intracellular binding and sequestration rather than ATP7B‑mediated drug efflux.
000000556 540__ $$fCC BY
000000556 542__ $$fIn copyright - single owner
000000556 650__ $$aIntestines$$020963
000000556 650__ $$aAdenosine Triphosphatases$$014245
000000556 650__ $$aOvary$$023388
000000556 650__ $$aHomeostasis$$020285
000000556 650__ $$aOvarian Neoplasms$$023386
000000556 650__ $$aCopper$$017086
000000556 691__ $$aSchool of Medicine$$041369
000000556 692__ $$aDepartment of Biochemistry and Molecular Biology$$041396
000000556 7001_ $$aZuzel, Vesna$$uOregon Health and Science University$$041354
000000556 7201_ $$aLutsenko, Svetlana$$uOregon Health and Science University$$041354$$7Personal$$eMentor
000000556 8564_ $$933fa0c17-2ef6-4ad2-8718-008463cf9056$$s1200818$$uhttps://digitalcollections.ohsu.edu/record/556/files/557_etd.pdf$$ePublic$$277284d2e58b55a0437d99f47817f306e$$31
000000556 905__ $$a/rest/prod/9p/29/09/35/9p2909353
000000556 909CO $$ooai:digitalcollections.ohsu.edu:556$$pstudent-work
000000556 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000556 980__ $$aTheses and Dissertations