TY  - GEN
AB  - The Portland Alcohol Research Center (PARC) was established to investigate the genetic basis of alcohol dependence.  One line of inquiry utilizes mouse strains that are widely divergent in alcohol‐related behaviors.  Decades of genetics research comparing mouse strains has identified many regions of the genome associated with such quantitative traits.  These regions are called Quantitative Trait Loci (QTLs).  Microarrays have been used to identify which genes within the QTLs are differentially expressed and are therefore potentially causal; however, genetic variants that affect probe hybridization lead to many false conclusions.  Here, we used quantitative proteomics to compare brain striata between two mouse strains for which abundant QTL and transcriptomic data is available.  The primary aims of this research were to (1) identify differentially expressed proteins that lie within QTLs and are therefore candidate causal proteins, (2) determine if genetic variants also lead to spurious results in quantitative proteomics, and (3) compare transcriptomic and proteomic datasets to determine their agreement.
AD  - Oregon Health and Science University
AU  - Fei, Suzanne
DA  - 2011
DO  - 10.6083/M4125QMG
DO  - DOI
ED  - McWeeney, Shannon
ED  - David, Larry
ED  - Boudreau, Eilis
ED  - Advisor
ED  - Advisor
ED  - Advisor
ID  - 587
KW  - Quantitative Trait Loci
KW  - Alcohol Drinking
KW  - Genomics
KW  - Proteomics
KW  - Genetics
KW  - Amino Acid Substitution
KW  - Ethanol
KW  - Quantitative Trait Loci
KW  - Alcoholism
KW  - qtl genes
L1  - https://digitalcollections.ohsu.edu/record/587/files/588_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/587/files/588_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/587/files/588_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/587/files/588_etd.pdf
N2  - The Portland Alcohol Research Center (PARC) was established to investigate the genetic basis of alcohol dependence.  One line of inquiry utilizes mouse strains that are widely divergent in alcohol‐related behaviors.  Decades of genetics research comparing mouse strains has identified many regions of the genome associated with such quantitative traits.  These regions are called Quantitative Trait Loci (QTLs).  Microarrays have been used to identify which genes within the QTLs are differentially expressed and are therefore potentially causal; however, genetic variants that affect probe hybridization lead to many false conclusions.  Here, we used quantitative proteomics to compare brain striata between two mouse strains for which abundant QTL and transcriptomic data is available.  The primary aims of this research were to (1) identify differentially expressed proteins that lie within QTLs and are therefore candidate causal proteins, (2) determine if genetic variants also lead to spurious results in quantitative proteomics, and (3) compare transcriptomic and proteomic datasets to determine their agreement.
PB  - Oregon Health and Science University
PY  - 2011
T1  - Integrating genetics and protemics to study alcohol-drinking behavior
TI  - Integrating genetics and protemics to study alcohol-drinking behavior
UR  - https://digitalcollections.ohsu.edu/record/587/files/588_etd.pdf
Y1  - 2011
ER  -