000000601 001__ 601
000000601 005__ 20260410124449.0
000000601 0247_ $$2DOI$$a10.6083/M46M34S0
000000601 037__ $$aETD
000000601 245__ $$aAn analysis of nerve growth factor-dependent differentiation and neurite outgrowth in the PC12 cell model system
000000601 260__ $$bOregon Health and Science University
000000601 269__ $$a2008
000000601 336__ $$aDissertation
000000601 502__ $$bPh.D.
000000601 502__ $$gNeuroscience
000000601 520__ $$aThe PC12 cell line is a well‑established model for studying nerve growth factor (NGF)–dependent neuronal differentiation, characterized by cell cycle exit and neurite outgrowth. This work investigates the signaling pathways and transcriptional mechanisms underlying NGF‑induced differentiation. Using pharmacological and molecular approaches, the study demonstrates that neurite outgrowth requires both ERK‑dependent and ERK‑independent pathways, including PI3K and Src family kinases, with sustained ERK activation necessary for maximal effects. The role of ERK signaling in regulating the transcription factor c‑fos is further defined, identifying specific phosphorylation events required for c‑fos stability and transactivation. Together, these findings clarify how NGF signaling coordinates intracellular pathways and gene regulation to drive neuronal differentiation in the PC12 model.
000000601 540__ $$fCC BY
000000601 542__ $$fIn copyright - single owner
000000601 650__ $$aPC12 Cells$$029345
000000601 650__ $$aNerve Growth Factor$$032821
000000601 650__ $$aCell Differentiation$$016279
000000601 650__ $$aPheochromocytoma$$023980
000000601 650__ $$aNeuronal Outgrowth$$011790
000000601 691__ $$aSchool of Medicine$$041369
000000601 692__ $$aVollum Institute$$041509
000000601 7001_ $$aPellegrino, Michael$$uOregon Health and Science University$$041354
000000601 7201_ $$aStork, Phillip$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor$$eMentor
000000601 8564_ $$9500317a3-111b-4ae4-bae5-5db87c3a4568$$s3261727$$uhttps://digitalcollections.ohsu.edu/record/601/files/602_etd.pdf$$ePublic$$2579d8132e905db38fcdbde57c769cffb$$31
000000601 901__ $$a<p>These documents are archival records. They are retained for historical reference only. </p><p><b>Need an accessible version? Use the ‘Get Accessible Copy’ link above.</b></p>
000000601 905__ $$a/rest/prod/4f/16/c2/84/4f16c2849
000000601 909CO $$ooai:digitalcollections.ohsu.edu:601$$pstudent-work
000000601 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000601 980__ $$aTheses and Dissertations