000000605 001__ 605
000000605 005__ 20260410124449.0
000000605 0247_ $$2DOI$$a10.6083/M4PN93M9
000000605 037__ $$aETD
000000605 245__ $$aRegulation of c-Myc phosphorylation, protein stability and oncogenic activity in breast cancer
000000605 260__ $$bOregon Health and Science University
000000605 269__ $$a2011
000000605 336__ $$aDissertation
000000605 502__ $$bPh.D.
000000605 520__ $$aThe c‑Myc oncoprotein is frequently dysregulated in human cancers, and its functional regulation and degradation are critical targets for therapeutic intervention. This study uses Saccharomyces cerevisiae as a model system to investigate c‑Myc phosphorylation, stability, and protein interactions. c‑Myc was shown to undergo sequential, interdependent phosphorylation at threonine 58 and serine 62 in yeast, mirroring regulation observed in mammalian cells and affecting protein stability. Yeast orthologs of known mammalian regulatory proteins similarly influenced these phosphorylation events, validating yeast as a relevant model. A yeast two‑hybrid screen identified ten novel c‑Myc interacting proteins, including HBP1, a tumor suppressor. HBP1 was found to bind c‑Myc and inhibit its transcriptional activity, providing new insight into c‑Myc regulation and tumor suppression.
000000605 540__ $$fCC BY
000000605 542__ $$fIn copyright - single owner
000000605 650__ $$aBreast Neoplasms$$015809
000000605 650__ $$aSignal Transduction$$028380
000000605 650__ $$aProto-Oncogene Proteins c-myc$$029021
000000605 650__ $$aProtein Stability$$038363
000000605 650__ $$aRepressor Proteins$$025328
000000605 650__ $$aBreast$$015806
000000605 6531_ $$acancer
000000605 6531_ $$aaxin
000000605 691__ $$aSchool of Medicine$$041369
000000605 692__ $$aDepartment of Molecular and Medical Genetics$$041428
000000605 7001_ $$aZhang, Xiaoli$$uOregon Health and Science University$$041354
000000605 8564_ $$93fa03977-ef51-4bdd-99f4-526732efc039$$s3088888$$uhttps://digitalcollections.ohsu.edu/record/605/files/606_etd.pdf$$ePublic$$237c5f7e4c890768aef68dc54ccfbd7c4$$31
000000605 901__ $$a<p>These documents are archival records. They are retained for historical reference only. </p><p><b>Need an accessible version? Use the ‘Get Accessible Copy’ link above.</b></p>
000000605 905__ $$a/rest/prod/34/84/zg/96/3484zg969
000000605 909CO $$ooai:digitalcollections.ohsu.edu:605$$pstudent-work
000000605 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000605 980__ $$aTheses and Dissertations