TY  - THES
AB  - Manganese (III, IV) oxides are key environmental oxidants, yet the enzymatic mechanisms of Mn(II) oxidation in Pseudomonas putida GB-1 remain unclear. This study localized Mn(II)-oxidizing activity to the outer membrane and identified a multi-copper oxidase (Mco) via MS/MS, though deletion of mco did not abolish activity, indicating multiple enzymes are involved. Reactive oxygen species, including superoxide and hydrogen peroxide, were shown to play essential roles in catalysis. Two hypothetical outer membrane proteins (2552, 2553) were found critical for Mn(II) oxidation and associated with MnxG, suggesting a complex multi-protein system. These findings advance understanding of bacterial manganese oxidation pathways.
AD  - Oregon Health and Science University
AU  - Buzzo, John
DA  - 2011
DO  - 10.6083/M41Z42C2
DO  - DOI
ED  - Tebo, Bradley
ED  - Advisor
ID  - 610
KW  - Pseudomonas putida
KW  - Pseudomonas
KW  - Manganese
KW  - Mass Spectrometry
KW  - Oxidoreductases
KW  - Reactive Oxygen Species
KW  - Membrane Proteins
KW  - Bacterial Outer Membrane Proteins
KW  - Oxidation-Reduction
KW  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
KW  - oxidation-reduction reaction
KW  - ms/ms spectroscopy
KW  - oxidases
L1  - https://digitalcollections.ohsu.edu/record/610/files/611_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/610/files/611_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/610/files/611_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/610/files/611_etd.pdf
N2  - Manganese (III, IV) oxides are key environmental oxidants, yet the enzymatic mechanisms of Mn(II) oxidation in Pseudomonas putida GB-1 remain unclear. This study localized Mn(II)-oxidizing activity to the outer membrane and identified a multi-copper oxidase (Mco) via MS/MS, though deletion of mco did not abolish activity, indicating multiple enzymes are involved. Reactive oxygen species, including superoxide and hydrogen peroxide, were shown to play essential roles in catalysis. Two hypothetical outer membrane proteins (2552, 2553) were found critical for Mn(II) oxidation and associated with MnxG, suggesting a complex multi-protein system. These findings advance understanding of bacterial manganese oxidation pathways.
PB  - Oregon Health and Science University
PY  - 2011
T1  - New insights in Mn(II) oxidation in Pseudomonas putida GB-1
TI  - New insights in Mn(II) oxidation in Pseudomonas putida GB-1
UR  - https://digitalcollections.ohsu.edu/record/610/files/611_etd.pdf
Y1  - 2011
ER  -